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剂量依赖的X射线辐射诱导的旁观者效应在MCF-7乳腺癌细胞中

Beatriz Allyne D Carandang¹, Teresa Sy-Ortin², John Paul C Cabahug³

¹University of the Philippines - Manila, Padre Faura Manila, Metro Manila, 1000, Philippines; University of Santo Tomas, Sampaloc Manila, Metro Manila, 1008, Philippines.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

|July 27, 2025

概括

电离辐射暴露可以在未被辐射的细胞中引发辐射诱导的旁观者效应 (RIBE). 这项研究表明,来自辐射的MCF-7癌细胞的介质显著降低了旁观细胞的活力和克隆生成能力,在较高的辐射剂量下,效应会增加.

科学领域:

辐射生物学辐射生物学
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

新出现的证据表明,电离辐射可以在非目标细胞中诱导生物效应,称为辐射诱导旁观者效应 (RIBE).
RIBE的表现和严重程度受到各种因素的影响,包括辐射类型,剂量和细胞系. 了解这些影响对于放射治疗和辐射保护至关重要.

研究的目的:

通过使用台式X射线照射器研究MCF-7乳腺癌细胞中辐射诱导的旁观者效应 (RIBE).
确定RIBE对细胞活力和克隆性潜力的剂量依赖关系.

主要方法:

培养MCF-7细胞并暴露于急性剂量的X射线辐射 (0-12 Gy).
从照射细胞 (ICM) 转移到非照射细胞中,以诱导旁观者效应.
细胞活力和殖民地形成测定在移植后24小时进行,以评估生物反应.

主要成果:

从被辐射的MCF-7细胞中暴露于ICM显著降低了受体非辐射细胞的活力和克隆性能力.
观察到的旁观者效应是剂量依赖的,在较高的辐射剂量 (3.36 Gy - 12 Gy) 中观察到的效应更为明显.

结论:

被辐射的MCF-7细胞在其介质中释放因子,这些因子在未被辐射的细胞中诱导显著的旁观者效应.
这些发现强调了辐射诱导的旁观者效应的剂量依赖性及其对癌症放射治疗和风险评估的潜在影响.

关键词:

乳腺癌乳腺癌乳腺癌旁观者效应是一种旁观者效应.细胞中的MCF-7细胞.这是X射线辐射的辐射.辐射治疗疗法辐射治疗疗法

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