剖析Kup和KimA对Enterococcus faecalis平衡的贡献
Giuliana Acciarri1,2, Maria Eugenia Taborra1,2, Fernan O Gizzi1,2
1Laboratorio de Fisiología y Genética de Bacterias Lácticas. Instituto de Biología Molecular y Celular de Rosario (IBR), Sede Facultad de Ciencias Bioquímicas y Farmacéuticas (FBioyF), Universidad Nacional de Rosario (UNR), Consejo Nacional de Ciencia y Tecnología (CONICET), Suipacha 590, Rosario, Argentina.
在吸收方面,Enterococcus faecalis使用KimA和Kup运输体,在离子稳态和应激适应方面具有不同的作用. 库普处理低和透应激,而基马处理高度.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- Enterococcus faecalis拥有多种不同的运输系统,包括Kdp,Ktr和Kup家族.
- 由于IS6770插入,JH2-2菌株缺乏Kdp复合体,并显示Kup基因表达发生变化.
- 在JH2-2中的kimA-kup位置包括用于金属运输,阻氧和Ca2+-ATPase的基因.
研究的目的:
- 为了描述E. faecalis JH2-2.中的kimA-kup位点.
- 研究KimA和Kup运输体的调节和功能作用.
- 了解它们对离子稳态和应激适应的贡献.
主要方法:
- 用RT-qPCR在不同pH和盐的条件下分析kimA和kup基因表达.
- 突变菌株 (KimA+,Kup+) 的构建和生长分析.
- 动力测试以确定的运输亲和力.
主要成果:
- 基马是单基斯特的,从植物性促进物转录出来,在酸性压力下升调.
- 在性条件下,kimA和kup表达与NaCl.增加.
- 库普+菌株比基马+菌株具有更高的亲和力.
- 基马调解高度的K+吸收;库普主导低度的吸收和性-性压力.
结论:
- 基马和库普在运输方面表现出功能差异.
- 这些载体在E. faecalis离子稳态和应激适应中起着不同的作用.
- 了解这些系统对于破译细菌生存机制至关重要.
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