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在感染流感的猪中单细胞抗原受体测序
Weihong Gu1,2, Darling Melany de Carvahlo Madrid1,2, Yuhan Wen1,2
1Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
Communications biology
|July 27, 2025
概括
新的原始剂使猪的单细胞免疫受体概况成为可能. 重新暴露于A型流感病毒的猪表现出扩展的T细胞克隆,这表明了潜在的病毒特异性反应,并强调了这种高通量测序方法的实用性.
科学领域:
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
- 兽医科学 兽医科学 兽医科学
背景情况:
- 单细胞RNA测序 (scRNAseq) 对于了解猪细胞表型至关重要.
- 免疫受体分析补充了scRNAseq,以全面了解免疫反应.
- 猪流感A病毒 (IAV) 感染模型对于研究宿主-病原体相互作用至关重要.
研究的目的:
- 开发和验证用于10x Genomics VDJ测序的猪特异性T细胞受体 (TCR) 和B细胞受体 (BCR) 丰富原料.
- 为了研究自然杀手T (NKT) 细胞对猪肺免疫受体谱的影响.
- 为了在IAV接种和感染的猪中纵向追踪T细胞的克隆扩张.
主要方法:
- 开发特定于猪的TCR和BCR丰富原料.
- 肺细胞和肺液T细胞的scRNAseq和VDJ测序.
- 对表达CD1D和缺乏CD1D的猪之间的免疫表达的比较.
- 从疫苗接种和感染的猪中对T细胞进行纵向采样和分析.
主要成果:
- 成功开发并验证了与10x Genomics兼容的猪特异性TCR和BCR丰富原料.
- 在所有研究的猪中确定了非常多样化的免疫谱.
- 在重新暴露于IAV的猪中观察到显著扩展的T细胞克隆类型与激活的表型,表明IAV反应的克隆.
- 证明了在纵向样本中跟踪克隆扩张的可行性.
结论:
- 开发的原始剂对猪中高通量单细胞TCR和BCR测序有效.
- 重新暴露于IAV会诱导猪肺中的扩大和激活的T细胞群.
- 这种方法为猪的适应性免疫反应提供了宝贵的见解,特别是在病毒感染后.
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