用Bacillus subtilis BS20生产抗真菌的工艺开发:纳米粒子补充,工艺优化和初步扩展研究
Sikhulile N Nzimande1, Isaac A Sanusi2, Kwasi Yobo3
1School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa.
Bioprocess and biosystems engineering
|July 27, 2025
概括
这项研究优化了Bacillus subtilis BS20用于增强抗真菌生产,为化学农药提供了可持续的替代品. 扩大规模的试验证明了成功的大规模生产潜力.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 密集型农业严重依赖化学农药,造成环境和作物生产风险.
- 生物控制剂,如Bacillus subtilis BS20,为化学农药提供环保和经济有效的替代品.
- 优化抗真菌生产对于开发可持续农业解决方案至关重要.
研究的目的:
- 为了增强Bacillus subtilis BS20的抗真菌生产.
- 使用建模和纳米催化剂优化发酵过程.
- 评估优化抗真菌生产工艺的扩展潜力.
主要方法:
- 在Bacillus subtilis BS20发酵的过程建模和优化.
- 研究的参数:葡萄糖度,化温度和时间.
- 纳米催化被探索,并通过使用恒定功率,雷诺兹数和螺旋尖速度来评估扩展.
主要成果:
- 在优化条件下 (11.5 g/L 葡萄糖,41°C,24h) 产生最大的抗真菌活性 (68 mm 抑制区域).
- 纳米粒子增加了生物质,但减少了抗真菌活性.
- 扩大到10升生物反应器保持了高的抗真菌活性 (65毫米的抑制区域).
结论:
- 过程优化显著提高了Bacillus subtilis BS20的抗真菌生产.
- 使用恒定倾斜速度的扩展策略对于大规模生产是有效的.
- 这项研究为Bacillus subtilis BS20作为生物控制剂的工业应用提供了基础.
更多相关视频
05:45Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
Published on: April 7, 2023
3.7K
06:08Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
380
相关概念视频
01:22Bioreactor Controls-III
67
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
67
01:14Scale-Up Processes
105
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
105
01:27Upstream Processing
97
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
97
01:27Production of Antibiotics
265
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
265
01:30Production of Pharmaceuticals
94
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
94
01:18Production of Biopesticides
108
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
108