相关实验视频

Updated: Sep 8, 2025

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

YTHDF3识别了DNAN6-甲基氨酸,并招募ALKBH1从基因组DNA中去除6mA

Xin-Hui Chen¹, Zi-Lu Wang¹, Jincui Yang¹

¹Biomedicine Research Center, Guangdong Provincial Key Laboratory of Major Obstetric Disease, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, 510150, Guangzhou, China.

The EMBO journal

|July 27, 2025

概括

YTHDF3增强了ALKBH1的作用.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

DNA N6 - - 甲基亚丁 (6mA) 是哺乳动物基因组中一个新兴的表观遗传标记.
ALKBH1是一种已知的6mA脱甲基酶,但其对双链DNA (dsDNA) 的活性仍在争论中.
需要进一步研究ALKBH1在调节基因组6mA水平,特别是dSDNA中的作用.

研究的目的:

研究YTHDF3在调节ALKBH1的6mA脱甲基酶活性中的作用.
为了确定YTHDF3是否能够从基因组DNA中去除6mA,包括dSDNA.
阐明YTHDF3在6mA上影响ALKBH1活动的机制.

主要方法:

生物化学测试以评估6mA脱甲基酶活性.
用于评估YTHDF3和ALKBH1与6mA修饰DNA的相互作用的DNA结合研究.
细胞测定以确认YTHDF3在6mA调节中的体内功能.

主要成果:

YTHDF3显著增强了ALKBH1在各种DNA结构上的6mA脱甲基酶活性,包括dsDNA.
YTHDF3优先与6mA修饰的DNA结合,并将ALKBH1招募到这些部位.
通过ALKBH1.1,YTHDF3便于通过ALKBH1.3从人类基因组DNA中去除6mA.

结论:

YTHDF3充当了一种新的基因组DNA阅读器,专门识别6mA.
YTHDF3将ALKBH1招募到6mA位点,从而在基因组dSDNA中促进6mA脱甲基化.
这种相互作用突出了哺乳动物6mA表观遗传调节的新机制.

关键词:

在 ALKBH1 中,基因组 6mA 阅读器基因组DNA 6mA 的基因组.这就是YTHDF3的原因.dsDNADNADNA 的意思是什么

更多相关视频

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Published on: August 5, 2022

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

相关概念视频

Proofreading

Proofreading

Mismatch Repair

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

Base Excision Repair

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...

Overview of DNA Repair

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...