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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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相关实验视频

Updated: Sep 13, 2025

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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E3RC:一个逐步的计算协议,用于探索使用常规RNA-seq数据的增强器RNA表达和调节.

Hua Yu1, Yunjie Liu2, Zhouming Zhang3

  • 1The First Affiliated Hospital, School of Basic Medical Sciences and Institute of Biomedical Innovation, Jiangxi Medical College, Nanchang University, Nanchang 330031, China; Jiangxi Provincial Key Laboratory of Hematological Diseases, Department of Hematology, Nanchang University, Nanchang 330031, China; Jiangxi Provincial Key Laboratory of Tumor Biology, Nanchang University, Nanchang 330031, China.

STAR protocols
|July 27, 2025
PubMed
概括

一个新的计算框架,E3RC,可以从RNA测序数据中探索增强器RNA (eRNA) 表达和调节. 这种方法有助于在小鼠胚胎中识别eRNA及其发育变化.

关键词:
生物信息学是一种生物信息学.在RNAseqqq中使用.序列分析是指进行序列分析.

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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相关实验视频

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科学领域:

  • 基因组学就是基因组学.
  • 发展生物学 发展生物学
  • 计算生物学 计算生物学

背景情况:

  • 增强型RNAs (eRNAs) 在基因调节中起着至关重要的作用.
  • 分析eRNA表达和调节需要专门的计算工具.
  • 可以利用常规RNA测序 (RNA-seq) 数据来研究eRNAs.

研究的目的:

  • 介绍E3RC,用于分析增强器RNA (eRNA) 表达和调节的计算框架.
  • 为确定eRNA及其表达动态提供逐步指导方针.
  • 在小鼠植入前胚胎发育过程中表达eRNA的变化.

主要方法:

  • 开发E3RC计算框架.
  • 应用E3RC来分析公共RNA测序数据.
  • 在小鼠植入前胚胎中识别和描述eRNA.

主要成果:

  • 通过使用E3RC框架成功识别了eRNA.
  • 在不同发育阶段对eRNA表达模式的表征.
  • 洞察胚胎发生过程中由eRNAs介导的转录调节.

结论:

  • E3RC框架提供了一种可靠的方法,用于从RNA-seq数据中进行eRNA分析.
  • 这种方法有助于研究eRNA在发展过程中的功能.
  • 这些发现为进一步研究eRNA介导的基因调节提供了基础.