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样品制备和清理方法用于临床上下蛋白质组学.

Amal Mohamed Kamal1,2, Amy Carfagno3, Cynthia Nagy3

  • 1Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, USA.

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概括

顶向下蛋白质组学 (TDP) 允许针对个性化医学进行疾病特异性蛋白质形式分析. 本综述涵盖了样本准备和清理方法,以推进临床TDP工作流程和生物标志物发现.

关键词:
临床蛋白质组学 临床蛋白质组学过器辅助的样本准备方法纳米颗粒是如何形成的降水量 降水量 降水量固体相提取方法 固体相提取方法顶向下的蛋白质组学序列大小排除色谱学色谱学单固态阶段增强样品准备方法

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科学领域:

  • 临床蛋白质组学 临床蛋白质组学
  • 发现生物标志物的发现.
  • 个性化医疗是个性化的医疗.

背景情况:

  • 临床样本中的蛋白质形式分析有助于阐明疾病机制和生物标志物识别.
  • 顶向下蛋白质组学 (TDP) 为个性化医疗提供了潜力,但面临样本准备的挑战.

研究的目的:

  • 审查临床自上而下的蛋白质组学的最先进的样品制备和清理协议.
  • 为临床TDP选择有效和高效的样本处理方法提供信息.

主要方法:

  • 总结了有针对性的 (亲和/非亲和丰富) 和无针对性的 (基于凝的分化) 样本准备协议.
  • 审查线下和线上样品清理程序,包括透析,SPE,FASP,沉和SPPP用于淡化和清洗剂去除.

主要成果:

  • 确定适用于临床TDP的各种样品制备和清洁技术.
  • 强调了不同方法的有效性,以去除盐和洗剂,这对于蛋白质形式分析至关重要.

结论:

  • 临床TDP具有显著的潜力,可以识别传统测试遗漏的疾病特异性蛋白质形式.
  • 对可重复的临床TDP工作流程的标准化准则对于更广泛的采用和利用样本准备进步至关重要.