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RNA元素及其在植物中的生物技术应用

Filip Lastovka¹, Hadrien Peyret^2,3, George P Lomonossoff²

¹Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.

The New phytologist

|July 28, 2025

概括

本综述探讨了增强植物生物技术的RNA调控元素. 了解RNA结构是通过基因工程改善植物特征和蛋白质生产的关键.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
在RNA生物学,RNA生物学.

背景情况:

RNA 调节元素,包括 cis 和 trans 作用因子,对于工程植物具有改进的特征和高效的异质蛋白质生产至关重要.
这些RNA元素的功能是由它们的核酸序列和它们的动态高阶结构决定的.

研究的目的:

审查与植物生物技术当前或潜在应用的RNA调节元素.
讨论涉及翻译控制,转录稳定性,蛋白质共产,条件表达,RNA衰变和独立于帽子的启动的RNA元素.
要突出适用于植物内 (暂时/稳定转化植物) 和体外植物基表达系统的RNA元素,以及与塑基因表达相关的元素.

主要方法:

已建立和新兴的RNA调节元素的文献综述.
基于序列和更高阶结构的RNA元素功能分析.
根据它们在基因表达和生物技术应用中的作用对RNA元素的分类.

主要成果:

确定了控制植物翻译,稳定性和表达的多种RNA元素.
突出应用在转型植物和体外系统中,包括塑基因表达.
检查了具有潜在未来应用的RNA元素,从其他生物体和需要进一步研究的领域中汲取.

结论:

RNA调节元件是推动植物生物技术发展的多功能工具.
对RNA结构-功能关系的进一步研究将在作物改良和生物制造领域开启新的应用.
RNA元素的战略工程为优化植物基因表达和功能提供了显著的潜力.

关键词:

这是一个RNA元素RNA元素.在RNA周转率上,RNA周转率生物技术是生物技术.基因表达的基因表达方式植物植物植物植物植物.转录转录是一种转录.翻译翻译翻译翻译翻译翻译

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