通过糖化修饰来增强大米蛋白水解酸的抗氧化活性
Zhimin Zhang1, Xinjian Bi1, Liangjie Hu1
1School of Food Science and Bioengineering, Hunan Provincial Key Laboratory of Cytochemistry, Changsha University of Science and Technology, Changsha, China.
Frontiers in nutrition
|July 28, 2025
概括
糖化显著提高了大米蛋白水解酸的抗氧化能力. 这些增强的化合物显示出作为功能性食品成分对抗氧化应激的承诺.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 营养科学 营养科学
背景情况:
- 米蛋白是有价值的营养来源,但需要修改以提高其功能性质.
- 抗氧化剂活性对于防止由活性氧物种 (ROS) 引起的细胞损伤至关重要.
- 糖化是改善食品蛋白质生物活性的一种有前途的技术.
研究的目的:
- 调查糖化米蛋白水解剂 (RPH) 的抗氧化潜力.
- 为了评估用果糖,西洛斯和阿拉比诺斯修改的RPH的疗效.
- 评估体外和体外抗氧化能力.
主要方法:
- 米蛋白水解剂 (RPH) 是通过酶化水解来制备的.
- 梅拉德反应被用来用果糖,西洛斯和阿拉比诺斯对RPH进行糖化.
- 在体外抗氧化剂测定包括Fe2+化能力和激素清除 (DPPH,ABTS,基,超氧化物).
- 在体内研究中,使用斑马鱼模型来评估氧化损伤,反应性氧物种 (ROS),氨酸 (MDA),催化酶 (CAT) 和总超氧化物转化酶 (T-SOD).
主要成果:
- 用西洛斯 (RPH-X) 进行糖化,提高了2,2-二-1-皮克里基基 (DPPH•) 清除率12.75%.
- 在斑马鱼模型中,用阿拉比诺斯 (RPH-A) 进行糖化证明了显著的活性氧物种 (ROS) 抑制 (84.78%).
- 与未经修改的RPH相比,所有测试的糖化RPH都表现出更好的抗氧化能力.
结论:
- 糖化有效地增强了大米蛋白水解酸的抗氧化特性.
- 这些修改后的RPH具有作为食品中的功能成分的潜力.
- 这项研究支持使用糖化米蛋白作为具有显著抗氧化功效的饮食成分.
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