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颗粒细胞殖民地刺激因子以一种依赖于IFN的I型IFN方式保护于急性系统性阿尔法病毒病

Muddassar Hameed^1,2,3, Pallavi Rai^1,2, Md Shakhawat Hossain^1,2

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, United States.

Frontiers in immunology

|July 28, 2025

概括

颗粒细胞殖民地刺激因子 (G-CSF) 在控制诸如 chikungunya 等阿尔法病毒感染方面发挥着作用. 阻断其受体 (G-CSFR) 会使疾病恶化,但I型干扰素信号可以纠正这种情况,表明G-CSF是治疗标.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
分子生物学分子生物学

背景情况:

关节病原性α病毒,如 chikungunya (CHIKV) 和mayaro病毒 (MAYV),导致衰弱性关节疼痛,并与髓状细胞透和像粒细胞殖民地刺激因子 (G-CSF) 这样的高细胞因子有关.
由各种细胞产生的G-CSF通过其受体 (G-CSFR) 在髓状细胞上发出信号,促进它们的增殖和分化,特别是中性粒细胞.
虽然G-CSF在 chikungunya 疾病中升高,但其在关节性阿尔法病毒病原体中的具体作用尚不清楚.

研究的目的:

通过使用缺乏G-CSFR的小鼠,研究G-CSF信号在CHIKV和MAYV感染中的作用.

主要方法:

缺乏G-CSFR的小鼠 (G-CSFR^-/-) 和野生类型的 littermates 被感染了CHIKV和MAYV.
监测临床症状,体重变化和免疫细胞群 (单细胞,中性粒细胞).
评估了阻断I型干扰素 (IFN) 信号对G-CSFR^-/-小鼠疾病严重性的影响.

主要成果:

与野生型对照相比,G-CSFR^-/-小鼠在CHIKV和MAYV感染后表现出持续的体重减轻.
在G-CSFR^-/-小鼠中,在整个感染过程中观察到更高比例的炎症单细胞和减少的中性粒细胞数量.
阻断I型IFN信号改善了G-CSFR^-/-小鼠感染阿尔法病毒的体重减轻.

结论:

第一种类型的IFN信号传递对于G-CSFR介导的关节致病性阿尔法病毒疾病的控制至关重要.
G-CSF及其受体G-CSFR代表调节宿主免疫反应对抗CHIKV和MAYV感染的潜在治疗标.

关键词:

在G-CSF中使用.玛雅罗病毒 (Mayaro virus) 是一种病毒.造成关节炎的阿尔法病毒奇孔古尼亚病毒病毒.中性粒细胞中性粒细胞.

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