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一个跨王国T6SS RNase效应器,针对 prokaryotic 和宿主细胞进行致病的产生
Yudi Wang1, Yongdong Li2, Zonglan Yu1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Cell reports
|July 28, 2025
概括
我们发现了TseR,一种新的细菌类型VI分泌系统 (T6SS) RNase从Yersinia pseudotuberculosis的效应器. 这种多功能效应器向细菌和宿主细胞中的RNA,影响肠道微生物群和病原性.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 细菌的VI型分泌系统 (T6SS) 对于细菌间相互作用和病变发生至关重要.
- T6SS效应体,特别是RNases,仍然没有得到充分的研究,这限制了我们对其作用的理解.
研究的目的:
- 为了表征一种新的T6SS RNase效应剂,TseR,来自Yersinia伪结核病 (Yptb).
- 研究TseR在细菌竞争和宿主-病原体相互作用中的功能.
主要方法:
- 将TseR分泌和表征为针对单链RNA的双价位阴离子依赖RNase.
- 在接触依赖和接触独立的T6SS机制中评估TseR的杀伤活动.
- 用RNA测序来识别TseR在大肠杆菌中的全球RNA裂变目标.
- 在体内研究评估TseR在感染期间的作用,包括肠道微生物组的改变和真核细胞向.
主要成果:
- TseR是一种由Yptb的T6SS-3分泌的多功能跨王国效应剂,由TsiR抑制.
- TseR对单链RNA表现出RNase活性,并调解细菌杀死,OmpC有助于进入.
- RNA-seq确定了全球RNA目标,揭示了TseR对基因表达的影响.
- TseR通过调节肠道微生物群并直接影响宿主细胞来促进病原性.
结论:
- TseR是一种新的T6SS RNase效应剂,在细菌竞争和Yptb病变发生过程中发挥着重要作用.
- 这项研究阐明了Ntox44域蛋白在细菌与宿主相互作用中的功能.
- 结果提供了对T6SS效应器多样性和机制的见解.
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