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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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相关实验视频

Updated: Sep 13, 2025

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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通过高维度流细胞计,通过高维度流细胞计进行高效的桃体T卵泡辅助细胞处理和功能分析.

Qin Xu1, Lihong Shi1, Brian L P Dizon2

  • 1Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health.

Journal of visualized experiments : JoVE
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概括

这项研究详细介绍了人类桃体细胞隔离和培养的协议,这对于研究T毛囊辅助细胞 (Tfh) 至关重要. 它还介绍了Tfh细胞研究的流细胞计方法和数据分析技术.

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科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学

背景情况:

  • 毛囊T辅助细胞 (Tfh) 对于适应性幽默免疫至关重要,影响B细胞在感染,自身免疫和疫苗接种中的反应.
  • 了解Tfh细胞的发育和功能是疫苗设计和针对免疫相关疾病的向治疗的关键.

研究的目的:

  • 为收集,隔离,保存和培养人类桃体细胞提供全面的协议.
  • 为详细的Tfh细胞表征引入优化的光谱流细胞计画面板.
  • 证明高维数据分析对于发现治疗诱导的免疫细胞差异的有用性.

主要方法:

  • 人类桃体组织采集,细胞分离和体外培养.
  • 开发和优化两个光谱流细胞测量面板用于Tfh细胞表型化.
  • 对高维流细胞计数据进行无监督分析的应用.

主要成果:

  • 建立了人类桃体细胞处理的标准化协议.
  • 优化的流细胞计画面板能够对Tfh细胞和相关免疫群体进行深入的表征.
  • 无监督分析成功地确定了高维免疫细胞数据中的微妙,治疗诱导的变化.

结论:

  • 人类桃体细胞为研究Tfh细胞生物学和免疫反应提供了有价值的模型.
  • 提出的协议和流细胞计画面板促进了强大的Tfh细胞研究.
  • 高维数据分析是一个强大的工具,用于发现免疫细胞对刺激或治疗的反应的改变.