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在利比亚医院的格拉姆阴性细菌中,碳烯酶的分子特征和多样性

Khouloud Slimene¹, Salem K Almahjoub², Aldukali A Alkeskas³

¹Université Aix-Marseille, IRD, APHM, MEPHI, Faculté de Médecine et de Pharmacie, France.

Journal of infection in developing countries

|July 28, 2025

概括

抗微生物耐药性是一种全球性威胁. 这项研究在利比亚医疗机构中发现了耐卡巴胺的格拉姆阴性细菌,揭示了NDM-1和OXA-48等普遍存在的耐药基因.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
公共卫生公共卫生

背景情况:

抗生素耐药性 (AMR) 构成了重大的公共卫生挑战,特别是在发展中国家,由于广泛使用抗生素.
来自医疗保健机构的细菌中的抗生素耐药性基因 (ARG) 的特征对于有效的控制策略至关重要.

研究的目的:

在利比亚医疗保健机构中从临床和环境样本中分离的グラム阴性细菌中研究和描述抗生素耐药性基因.
确定特定的碳烯酶基因和其他耐药性机制的流行率.

主要方法:

从不同地点的不同来源收集了379个样本,其中210个产生了细菌生长.
从临床 (n=64) 和环境 (n=69) 样本中选择了133种耐卡巴胺的格兰阴性菌株.
利用抗生素敏感性测试,胆固醇耐药性测试 (UMIC方法) 和RT-PCR检测碳烯酶基因.

主要成果:

格拉姆阴性细菌对碳烯的敏感性较低.
在Enterobacteriaceae中,NDM-1是最常见的碳酶基因 (患者:n=26,环境:n=41),其次是blaOXA-48.
在 *Acinetobacter baumannii* (患者:n=8,环境:n=6) 和 *Pseudomonas aeruginosa* 耐药性相关的 OprD 基因变异中,blaOXA-23 是普遍存在的.

结论:

在利比亚的医疗保健机构中发现了多种耐卡巴胺的格拉姆阴性细菌.
该研究强调了各种卡巴酶和OprD变化的表达,作为关键的抵抗机制.

关键词:

格拉姆阴性细菌是一种细菌.利比亚利比亚利比亚利比亚抗微生物耐药性抗微生物耐药性碳烯质量质量问题临床样本临床样本医院环境医院环境

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