用于诊断传染病的分子检测的使用和管理:一个横截面调查
Caitlin Naureckas Li1,2,3, Jennifer A Blumenthal4,5,6, Anna C Sick-Samuels7,8
1Division of Infectious Diseases, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL, United States.
Journal of the Pediatric Infectious Diseases Society
|July 28, 2025
概括
血元基因组下一代测序被广泛用于诊断传染病. 然而,其使用频率在医疗机构之间有很大差异,这表明需要优化实施策略.
科学领域:
- 临床微生物学 临床微生物学
- 分子诊断学 分子诊断学
- 传染病流行病学 传染病流行病学
背景情况:
- 非定向分子测试正在成为传染病诊断的宝贵工具.
- 血元基因组下一代测序 (mNGS) 提供了一种广泛的方法来识别病原体.
研究的目的:
- 评估当前血甲基因组下一代测序的国家利用模式.
- 了解mNGS在临床实践中的使用频率和背景.
- 确定对管理和未来研究的感知需求.
主要方法:
- 在医疗保健提供者和实验室中进行了一项全国性调查.
- 该调查收集了关于血mNGS使用的采用和频率的数据.
- 受访者提供了关于实施挑战和未来方向的定性反.
主要成果:
- 血基因组下一代测序显示广泛采用,有89.8%的受访者报告其使用.
- 使用频率差异很大,从偶尔使用 (18.4%使用过几次) 到频繁使用 (>每周,9.6%).
- 受访者强调了测试管理的重要性,并建议未来研究的领域.
结论:
- 血mNGS越来越多地被整合到全国的传染病诊断中.
- 使用频率的变化表明需要标准化指南和优化实施.
- 进一步的研究和管理工作至关重要,以最大限度地提高血mNGS的临床效用.
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