使用二极管阵列探测器从生提取物中分离出来的生和shogaols的HPLC分析
Jinhui Liu1, Xiongjun Zuo1, Dan Wang1
1Analysis and Testing Center, South China Normal University, Guangzhou 510006, PR China.
概括
一种新的高性能液态色谱 (HPLC) 方法准确地分析生提取物中的生和shogaols. 这种具有成本效益的技术改进了现有的生分析质量控制方法.
科学领域:
- 分析化学 分析化学
- 药理学是指药理学,它是指药理学.
背景情况:
- 生和shogaols是生中的关键生物活性化合物.
- 准确的量化对于生质量控制和原产地分析至关重要.
- 现有的方法在分离和峰值识别方面面临挑战.
研究的目的:
- 开发一种简单,廉价和有效的HPLC方法来分析生醇和shogaols.
- 改进生提取物中这些化合物的区分和定量.
- 为现有质量控制方法提供可靠的替代方案.
主要方法:
- 使用二极管阵列探测器 (DAD) 的高性能液态染色学 (HPLC).
- 在C18柱上使用水和甲醇混合物进行线性梯度化.
- 通过溶剂和超临界CO2提取获得的生提取物的分析.
主要成果:
- 使用HPLC-DAD成功分离和检测醇和shogaols.
- 基于其独特的染色体对目标化合物的歧视.
- 准确和定量分析,克服INA方法的局限性 114.000.
- 已证明适用于不同的生提取方法.
结论:
- 开发的HPLC-DAD方法简单,便宜,并且非常有效.
- 这种方法提供了准确的量化和对gingerols和shogaols的区分.
- 它对生根茎切片的原产地分析有显著的前景.
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