口腔微生物组及其对呼出的呼吸的影响 挥发性分析 - 房间里的大象
Lorenzo S Petralia1, Anesu Chawaguta1, Veronika Ruzsanyi1
1Institute for Breath Research, Universität Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria.
Journal of breath research
|July 28, 2025
概括
呼出的呼吸含有健康生物标志物,但口腔微生物可以干扰. 建议采用鼻腔呼吸样本来准确分析内源性挥发物,避免错误的生物标志物识别.
科学领域:
- 生物医学科学 生物医学科学
- 分析化学 分析化学
- 微生物学 微生物学
背景情况:
- 呼出的呼吸挥发物 (EBV) 显示出作为健康和代谢状态的非侵入性生物标志物的潜力.
- 口服微生物产生的挥发性物质是EBV研究中的一个重要的混因素.
- 当前的研究往往忽视了口腔微生物群对呼气组成的影响.
研究的目的:
- 为了比较分析鼻腔和口腔中选择的挥发性物质的潮尾呼出的气息.
- 调查口腔微生物群对呼出的挥发性物质的影响.
- 确定最佳采样方法,可靠地发现内源性挥发性生物标志物.
主要方法:
- 这是一项涉及21名志愿者的比较横截面研究.
- 气色谱-离子移动性光谱 (GC-IMS) 用于挥发性化合物的检测.
- 主要成分分析 (PCA) 用于区分采样方法和确定关键的挥发性.
主要成果:
- 通过PCA观察到基于鼻腔和口腔采样方法的挥发性资料的清晰分离.
- 鉴定了四种独立于采样方法的挥发性物质 (乙,异烯,甲醇,2-) 和四种依赖性物质 (1-propanol,2-propanol,乙醇,乙).
- 鼻腔采样的挥发物对吸入途径 (口腔或鼻腔) 的影响很小.
结论:
- 在呼出的挥发性生物标志物研究中,对减轻口腔微生物群干扰至关重要.
- 如果不使用鼻腔采样,可能会导致口腔微生物挥发物被误认为内源生物标志物.
- 需要开发用户友好的鼻腔呼吸采集设备,以便广泛采用.
相关概念视频
Serum Laboratory Studies, Stool Test, Breath Test
476
Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...
476
Bacterial Flora of the Large Intestine
651
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
651
Applications of Molecular Taxonomy
109
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
109


