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Updated: Sep 13, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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开发一种基于DNA内核酶I-SceI的Cupriavidus necator无痕基因组编辑系统

Suk-Jin Oh1, Gaeun Lim1, Yebin Han1

  • 1Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.

Journal of biotechnology
|July 28, 2025
PubMed
概括

一个新的I-SceI同源重组 (HR) 系统简化了Cupriavidus necator中的基因工程,使得用于聚酸酸盐生产的精确基因编辑成为可能. 这种方法加速了用于合成生物学应用的菌株开发.

关键词:
基因删除是一种基因删除.基因插入 基因插入它们是PHA合成酶.拉尔斯托尼亚 (Ralstonia eutropha) 是一种缩的植物.

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