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相关概念视频

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
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Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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相关实验视频

Updated: Sep 13, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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基因序列数据治理和数据库工程的LISTEN原则.

Colin J Carlson1, Monica Granados2, Alexandra Phelan3

  • 1Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA. colin.carlson@yale.edu.

Nature genetics
|July 28, 2025
PubMed
概括
此摘要是机器生成的。

对遗传数据的访问和利益共享 (ABS) 义务可以与开放科学原则相兼容. 简单的数据库设计和治理原则,如LISTEN,确保在开放的科学数据库中实现ABS.

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Novel Sequence Discovery by Subtractive Genomics
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Novel Sequence Discovery by Subtractive Genomics

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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

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相关实验视频

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

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Novel Sequence Discovery by Subtractive Genomics

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科学领域:

  • 生物信息学是一种生物信息学.
  • 开放科学是一个开放的科学.
  • 法律信息学 法律信息学

背景情况:

  • 国际法律协议要求对基因序列数据进行访问和利益共享 (ABS).
  • 批评者认为,ABS与开放科学原则相冲突,并对开放数据库提出实施挑战.

研究的目的:

  • 通过展示ABS与开放科学的兼容性来解决批评.
  • 提出将ABS集成到开放科学数据库中的实际解决方案.

主要方法:

  • 分析开放科学原则和ABS法律框架.
  • 为数据库设计和治理制定六项原则清单 (LISTEN:授权,识别,监督,透明,强制执行,非排他性).
  • 探索LISTEN原则和FAIR数据共享原则之间的协同作用.

主要成果:

  • 对ABS与开放科学不兼容的批评源于误解.
  • LISTEN 检查列表为在数据库中实现 ABS 提供了一个实用的框架.
  • LISTEN原则可以补充现有的开放科学数据共享框架,如FAIR.

结论:

  • 通过深思熟虑的数据库设计和治理,ABS机制可以有效地整合到开放科学中.
  • 对于与ABS系统互动的平台,LISTEN原则提供了一个可扩展的解决方案.
  • 这种方法使法律义务与开放科学数据共享的道德相协调.