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巨细胞形成树状的伪足,以增强细菌的摄入

Changyuan Fan^1,2,3, Xinyi Huang^1,2, Jie Mei^1,2,3

¹Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 201203, Shanghai, China.

The EMBO journal

|July 28, 2025

概括

巨细胞形成树状伪脚 (DLP) 来增强感染期间的细菌吸收. 这种适应是由托尔类受体4 (TLR4) 信号驱动的,增强了对格拉姆阴性细菌的先天免疫防御.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
微生物学微生物学

背景情况:

巨细胞是关键的先天性免疫细胞,在感染期间具有适应性功能.
巨细胞形状变化及其免疫作用之间的联系尚未完全理解.

研究的目的:

为了研究巨细胞在格拉姆阴性细菌感染期间形成状伪足 (DLP) 的新型适应.
阐明DLP在增强宿主防御中的机制和功能意义.

主要方法:

使用了体外,微流体和体内感染模型.
研究了托尔类受体4 (TLR4) 和NF-κB信号通路.
分析了RhoGEF,ARHGEF3,Rhoa,actin和vimentin在DLP形成和功能中的作用.

主要成果:

发现严重的阴性细菌感染诱导了巨细胞DLP的快速形成.
证明DLP通过扩大巨细胞的搜索区域来增加细菌的吸收.
确定TLR4-NF-κB-ARHGEF3信号传递对于DLP形成和RhoA激活至关重要.
显示维门丁稳定DLP,而动因动力学驱动它们的延长.
证实,配备DLP的巨细胞可以降低沙门氏菌感染小鼠的细菌负载.

结论:

巨细胞的形态可塑性,特别是DLP的形成,是对严重细菌感染的快速适应性反应.
DLP显著增强细菌清除,并增强先天免疫力.
这项研究揭示了一种涉及TLR4-ARHGEF3-RhoA信号传递在巨介导宿主防御中的新奇机制.

关键词:

在Actin Filaments中使用.类似树的伪类动物.巨细胞是一个巨细胞.病原体的摄入病原体的摄入维门中介纤维维门中介纤维

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