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维生素D和脂聚糖合物共同诱导人体单细胞中独特的表观遗传和转录程序

Mariusz Jankowski¹, Emmi Hämäläinen^2,3, Mari Taipale^2,4

¹Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, ul. Trylińskiego 18, Olsztyn, 10-683, Poland.

Scientific reports

|July 29, 2025

概括

维生素D (1,25(OH) 2D3) 和脂多糖 (LPS) 在单细胞中协同重编程表观遗传和基因表达. 这揭示了新的维生素D标参与免疫细胞分化,而不仅仅是炎症.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

与病原体相关的分子模式,如脂多糖 (LPS) 触发免疫反应.
维生素D的活性形式,1α,25-二氧维生素D3 [1,25(OH) 2D3],增强先天免疫力,但其机制尚未完全理解.

研究的目的:

调查1,25(OH) 2D3和LPS对THP-1单细胞表观基因组和转录基因组的联合作用.
确定维生素D在先天免疫和炎症中的作用背后的新型分子机制.

主要方法:

使用ATAC-seq进行表观遗传学分析,以评估染色质的可访问性.
使用RNA-seq进行转录组分析,以测量基因表达变化.
单细胞被原始化并用1,25(OH) 2D3,LPS或它们的组合刺激.

主要成果:

与1,25(OH) 2D3和LPS的同时刺激诱导了染色质可访问性的显著协同变化,其中81%的变化是该组合独有的.
鉴定出JUN/FOS转录因子是协同反应的关键调节者.
在1,25(OH) 2D3原始化下,331个基因显示出协同表达变化,其中包括264个参与单细胞和T细胞分化的新型维生素D标.

结论:

维生素D通过协同的表观遗传和转录重编程来调节炎症,以应对细菌刺激.
已经确定了影响免疫细胞分化的新型维生素D标,超出了经典的炎症途径.

关键词:

染色体的可访问性染色体的可访问性免疫挑战是一个挑战.脂聚糖类糖化物脂聚糖类糖化物响应性基因是一种响应性基因.转录组转录组就是一个转录组.维生素D 维生素D是一种维生素.

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