相关实验视频

Updated: Sep 13, 2025

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Published on: January 5, 2016

在患者衍生的外体蛋白中对HIV1宿主蛋白-蛋白相互作用网络进行分析:对病理生理学和先天免疫通路的影响

Noor Fatima¹, Mirza Sarwar Baig², Aman Haider Rizvi²

¹Department of Molecular Medicine, School of Interdisciplinary Sciences and Technology, Jamia Hamdard, New Delhi, 110062, India.

Virology journal

|July 29, 2025

概括

这项研究揭示了HIV-1患者的外体如何携带宿主和病毒蛋白质,影响免疫路径,并为艾滋病毒疾病进展提供潜在的治疗生物标志物.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
细胞生物学细胞生物学

背景情况:

人类免疫缺陷病毒 (HIV) 通过向宿主免疫系统,导致获得性免疫缺陷综合征 (AIDS).
外体,小囊泡参与细胞间通信,由HIV-1感染细胞释放.
了解宿主-病原体相互作用对于开发有效的HIV疗法至关重要.

研究的目的:

研究外体源蛋白在HIV病理生理学和宿主免疫反应中的作用.
阐明艾滋病毒感染中由外体介导的细胞间通信机制.
为了识别潜在的基于HIV的外体生物标志物theranostics.

主要方法:

从艾滋病毒患者血清中分离出外体.
液体染色学-双重质谱法 (LC-MS/MS) 用于蛋白质的识别.
病毒和宿主蛋白之间的蛋白质与蛋白质相互作用 (PPI) 网络分析.

主要成果:

鉴定出了12种外体源宿主蛋白,其中5种参与托尔类受体 (TLR) 信号传递,炎细胞激活,亡抑制和自.
发现CDH5,ENO1,OGT和TRAF6蛋白质参与多种免疫通路.
艾滋病毒-1蛋白质 (Gag,Gag-pol,Env,Nef) 与许多参与天生的免疫反应的人类蛋白质相互作用.

结论:

外体源蛋白在HIV-1病理生理学和免疫调节中起着重要作用.
这些发现提供了对宿主-病原体相互作用和艾滋病毒感染的先天免疫力的洞察.
基于外体的生物标志物显示出对艾滋病毒治疗应用的前景.

关键词:

生物格里德 4.4 版本外基因组是外基因组的组成部分.人类免疫缺陷病毒-1型.炎症酶激活的激活天生的免疫反应.蛋白质蛋白质相互作用在SIGNOR版本3.0中使用.

更多相关视频

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

Published on: March 3, 2017

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...