从口腔粘膜和边缘膜中获得的细胞中血管化和炎症调节者的不同表达
Eleni Voukali1, Joao Victor Cabral1, Natalia Smorodinova1
1Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague, 128 00 Prague, Czech Republic.
Bioengineering (Basel, Switzerland)
|July 29, 2025
概括
对肢体干细胞缺乏症 (LSCD) 的培养口腔粘膜上皮细胞移植 (COMET) 显示,与肢体上皮细胞 (LEC) 相比,由于口腔粘膜上皮细胞 (OMEC) 炎症基因表达增加,新血管化更高.
科学领域:
- 眼科医生 眼科 眼科
- 再生医学是一种再生医学.
- 分子生物学分子生物学
背景情况:
- 双边四肢干细胞缺乏症 (LSCD) 用培养的口腔粘膜上皮细胞移植 (COMET) 来治疗.
- 与肢体干细胞 (LESC) 移植相比,COMET表现出增加的新血管化,用于单方面LSCD.
- 了解口腔粘膜上皮细胞 (OMEC) 和四肢上皮细胞 (LEC) 之间的分子差异对于改善移植结果至关重要.
研究的目的:
- 研究与血管化和炎症相关的OMEC和LEC的体外基因表达特征.
- 确定有助于在COMET与LESC移植中观察到的差异性新血管化的分子因素.
主要方法:
- 口腔粘膜上皮细胞 (OMECs) 和四肢上皮细胞 (LECs) 使用常规 (COM) 或无异生菌 (XF) 介质在纤维素基质上进行培养.
- 用实时定量PCR进行基因表达分析.
- 多因素线性模型被用来分析基因表达数据.
主要成果:
- 在LEC中,亲血管性基因 (如AGR2,ANGPTL2) 和抗血管性基因 (如TIMP3,SERPINF1) 的表达更高.
- OMECs表现出更高的亲血管性FGF2和抗血管性COL18A1.1的表达.
- 与LEC相比,OMECs表现出关键免疫调节基因的显著增加,包括IL1B,IL6,TNF,CXCL10和IL1RN,与LEC相比.
结论:
- 在OMEC和LEC之间存在明显的转录特征,特别是在血管新生和炎症介质方面.
- 在OMEC中炎症基因的高表达可能有助于在COMET后观察到的新血管化的增加.
- 这些发现为COMET中新血管化的分子基础提供了洞察力,并建议治疗调制的目标.
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