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甲状腺癌诊断的分子标记:来自MAPK途径基因表达分析的见解

Breno Pupin¹, Ramon Varella Diniz¹, Maricilia Silva Costa²

¹Laboratório de Genética Molecular-GeneLab, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba-UNIVAP, Avenida Shishima Hifumi 2911, Urbanova, São José dos Campos 12244-000, SP, Brazil.

|July 29, 2025

概括

这项研究确定了MAPK途径中的关键基因,如CCNA1和SFN,作为甲状腺癌的潜在诊断标记物,提高了乳头和卵泡癌的诊断准确性.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
在瘤学瘤学.

背景情况:

在全球范围内,甲状腺癌的发病率正在上升,这对公众健康构成了重大挑战.
目前用于甲状腺癌的诊断方法往往缺乏足够的灵敏度和特异性.
分子分析为改善甲状腺癌分化和诊断提供了一种有前途的方法.

研究的目的:

为了确定用于增强甲状腺癌诊断的新型分子标记物.
分析基因激活蛋白激酶 (MAPK) 路径基因的表达,以确定诊断潜力.
提高区分甲状腺癌的敏感性和特异性.

主要方法:

在甲状腺样本中使用PCR阵列对46个MAPK通路基因的差异基因表达分析.
使用定量逆转录PCR (RT-qPCR) 验证已识别的标记物.
使用癌症基因组图谱 (TCGA) 的基因表达数据的分析.

主要成果:

在甲状腺癌中,CCNA1,CDKN1C,CREB1,FOS,HSPA5,JUN,MAP2K6和SFN基因显示出差异性表达.
CCNA1和SFN显示出作为皮肤状甲状腺癌的诊断标记物的潜力.
减少CDKN1C,FOS和JUN的表达可能有助于区分卵泡癌.

结论:

CCNA1,CDKN1C,FOS,JUN和SFN被确定为甲状腺癌的有前途的诊断生物标志物.
这些分子标记物有可能有助于甲状腺癌患者的临床决策.
进一步的研究可以验证这些标记物在甲状腺病理学中的常规诊断用途.

关键词:

通过MAPK路径.诊断诊断诊断的诊断诊断诊断诊断的诊断基因表达的基因表达方式甲状腺癌是甲状腺癌.

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