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Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

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相关实验视频

Updated: Sep 13, 2025

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

Published on: January 19, 2019

单细胞转录组分析揭示了肺腺癌进展中的关键调节器和信号通路.

Jialu Ma^1,2, Caleb McQuay³, John Talburt²

¹MidSouth Bioinformatics Center and Joint Bioinformatics Graduate Program, University of Arkansas at Little Rock, Little Rock, AR 72204, USA.

|July 29, 2025

概括

单细胞测序揭示了晚期肺腺癌 (LUAD) 中明显的上皮细胞变化. 这些发现在瘤微环境中确定了新的标记物和通信通路,以改善LUAD治疗.

关键词:

肺腺癌的进展情况信号通道的信号通道.一个单细胞RNA测序.转录调节器是一个转录调节器.

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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

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Published on: September 20, 2016

相关实验视频

Last Updated: Sep 13, 2025

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

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Published on: January 19, 2019

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

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Published on: March 30, 2019

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

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Published on: September 20, 2016

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

肺腺癌 (LUAD) 是癌症死亡的主要原因,需要新的治疗策略.
单细胞RNA测序 (scRNA-seq) 为了解癌症的细胞异质性提供了前所未有的分辨率.
LUAD的复杂性往往被传统的批量转录组分析所掩盖.

研究的目的:

通过使用scRNA-seq. 来研究在LUAD进展期间细胞类型特定的转录组变化.
识别特定阶段的基因标记物及其调节网络.
分析瘤微环境 (TME) 中跨LUAD阶段的细胞间通信动态.

主要方法:

来自多个LUAD患者队列的scRNA-seq数据的综合分析.
聚类,血统轨迹和转录监管网络的重建.
构建细胞间通信网络,绘制TME信号的地图.

主要成果:

皮质细胞在IV阶段的LUAD中表现出不同的转录特征,与免疫细胞或树皮细胞不同.
确定了一组特定阶段的上皮细胞基因标记物,与生存率和TME组成相关.
关键的转录因子 (ATF3,ATF4,HSF1,KLF4,NFIC) 被认为是上游监管者.
晚期的LUAD表现出皮质衍生信号的增加和免疫衍生信号的减少.
确定了Wnt,PTN和PDGF通路在通过细胞与细胞通信的LUAD进展中至关重要.

结论:

这项研究提供了一个单细胞图的LUAD进展,强调上皮细胞调节和TME通信.
发现了具有预后和治疗潜力的新型分子标记物和调节机制.
这些发现为更精确的LUAD治疗铺平了道路,通过准已识别的途径和调节器.