通过ScnR1介导的竞争性DNA结合和反抑制 调节Streptomyces caniferus中的政府菌素生物合成
Haoran Shi1,2, Jiabin Wang1,2, Xuedong Zhang1,2
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
Biology
|July 29, 2025
概括
一个新发现的转录调节器,ScnR1,通过竞争性地与关键促进体结合,微调了Streptomyces中的 guvermectin生物合成. 这一发现揭示了二次新陈代谢的复杂调节网络,有助于未来的菌株工程,以提高天然产品的产量.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 瓜尔梅克是一种Streptomyces衍生的化合物,具有植物生长调节剂和抗菌性质.
- 它的生物合成是由GvmR和GvmR2控制的,但远端调节者是未知的.
研究的目的:
- 识别和表征涉及于瓜维美克生物合成的远端转录调节体 (TRs).
- 阐明控制Streptomyces中的政府菌素生产的调控机制.
主要方法:
- 识别ScnR1,一个LacI家族TR,并通过GvmR激活它.
- 通过过度表达,对ScnR1通过过度表达对nguvermectin生物合成的影响的分析.
- 研究ScnR1的DNA结合到目标促进体 (gvmR,gvmA,O1) 的研究.
- 在ScnR1和GvmR2.2之间的反循环的特征.
主要成果:
- 位于远部的ScnR1被GvmR识别并激活.
- 过度表达ScnR1显著降低了州政府素的产生.
- ScnR1 竞争性地与促进体结合,抑制了政府菌素 BGC 转录.
- 在ScnR1和GvmR2.2之间存在一个反循环.
结论:
- 揭示了一种涉及ScnR1和GvmR2的多层级调节机制,用于涉及ScnR1和GvmR2的政府素生物合成.
- 通过竞争性结合和反,LacI家族TRs通过竞争性结合和反微调二次新陈代谢.
- 结果提供了关于Streptomyces二次代谢和菌株工程的见解,以增强天然产品.
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