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通过CRISPR/Cas9精确编辑chNHE1基因产生ALV-J抗性原生殖细胞

Xinyi Zhou¹, Ruyu Liao¹, Min Tan¹

¹College of Animal Science and Technology, Southwest University, Chongqing 400715, China.

Animals : an open access journal from MDPI

|July 29, 2025

概括

通过修改chNHE1受体,CRISPR/Cas9基因编辑产生了对禽类白血病病毒亚组J (ALV-J) 耐药的原生殖细胞. 这一突破为开发抗病家禽和推进鸟类基因编辑提供了新的战略.

科学领域:

* 分子生物学 * 分子生物学
* 遗传学遗传学是一门学科
* 动物科学动物科学

背景情况:

* 禽类白血病病毒亚组J (ALV-J) 导致家禽免疫抑制和瘤,影响家禽业.
*ALV-J感染依赖于与肉-交换机1型 (chNHE1) 受体的结合,特别是在酸残留物38 (W38) 的结合.

研究的目的:

* 在原生殖细胞 (PGC) 中开发一个体外基因编辑系统,以赋予对ALV-J的抵抗力.
*利用CRISPR/Cas9技术准和修改肉PGC中的chNHE1受体的W38位点.

主要方法:

* 为CRISPR/Cas9输送构建一个双晶状病毒载体,针对chNHE1.1的W38位点.
*用lentiviral载体感染的PGC,并验证基因编辑效率.
*ALV-J感染测定基因编辑和控制PGC,包括env基因表达分析和p27抗原检测.

主要成果:

*CRISPR/Cas9系统有效地准了肉PGC中的W38位点,并观察到高传染率.
*经基因编辑的PGC在ALV-J感染后的48小时和72小时没有检测到可检测的病毒基因表达.
*ELISA测试证实,感染7天后编辑的PGC中没有p27抗原,表明耐药性.

结论:

*使用CRISPR/Cas9技术成功生成了ALV-J抗性基因编辑PGCs.
* W38修饰的chNHE1受体有效地阻止ALV-J的进入,显示出显著的抗病毒功效.
*这项研究为抗病禽养殖提供了一种新的策略,并推进了鸟类基因编辑的应用.

关键词:

这就是CRISPR/Cas9的作用.鸟类白血病病毒亚组J (ALV-J)这就是为什么chNHE1 基因编辑基因编辑原始生殖细胞 (PGCs) 是一种

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