淋巴激素和生长因子在调节Ras的作用在生长过程中在猪的积卵子复合体中成熟
Eunju Seok1, Minyoung Son1, Seunghyung Lee1
1College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
Animals : an open access journal from MDPI
|July 29, 2025
概括
猪的毛囊发育包括Ras GTPases. 毛囊刺激激素 (FSH) 调高R-Ras表达,促进卵细胞成熟和细胞增殖.
科学领域:
- 生殖生物学 生殖生物学
- 细胞信号传输 细胞信号传输
- 分子内分泌学分子内分泌学
背景情况:
- 卵细胞和累积细胞的成熟涉及由淋巴激素和生长因子调节的复杂信号通路.
- 包括Ras GTPases在内的小G蛋白在介导卵细胞积复合体 (COC) 生理变化期间的细胞反应中发挥着关键作用.
- 了解Ras家族成员在猪COC成熟中的特定作用对于生殖科学至关重要.
研究的目的:
- 调查性腺激素 (FSH,LH) 和生长因子 (EGF) 在猪COC成熟期间对Ras及其GTPases的影响.
- 确定R-Ras在调节COC发育过程中的细胞增殖和基因表达中的特定作用.
- 确定FSH,LH和EGF的最佳度,以增强R-Ras表达和COC增殖.
主要方法:
- 猪不成熟的COC被培养了44小时,FSH,LH或EGF的度各不相同.
- 采用RT-PCR分析了Ras亚家族成员 (H-Ras,K-Ras,N-Ras,R-Ras),GTPases (RASA1,SOS1) 和增殖标志物 (ERK,CCNB1,Cdc2) 的mRNA表达水平.
- 进行了定量分析,以评估FSH,LH和EGF对基因表达和细胞增殖的剂量依赖作用.
主要成果:
- 与其他Ras亚家族相比,在猪的COC成熟期间,R-Ras表达显著上调.
- 对于增强R-Ras表达和细胞增殖的最佳度被确定为0.5μg/mLFSH,10 IU/mLhCG和10 ng/mLEGF.
- 补充FSH独特地调高R-Ras和ERK表达,同时调低Cdc2,独立于LH和EGF的行动.
结论:
- 在猪COC成熟过程中,FSH在调节R-Ras表达方面发挥着至关重要的作用,从而促进细胞增殖.
- 这项研究阐明了R-Ras GTPase在卵细胞发育期间的FSH介导信号通路中的特定参与.
- 这些发现为了解Ras GTPase在猪毛囊环境的发展中的功能提供了基础知识.
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