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鉴定了哺乳动物calreticulin的新型抗菌功能
Yichao Ma1, Jiachen Liu1, Xinming Qin1
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Biomolecules
|July 29, 2025
概括
一种蛋白质,calreticulin,在山羊中表现出对大肠杆菌和P. multocida等细菌的抗菌活性. 这表明calreticulin可能是对抗呼吸道感染的新策略.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 卡尔雷蒂库林是一种已知的分子伴侣,参与平衡,蛋白质折叠和免疫调节.
- 新出现的证据表明,calreticulin具有抗菌功能,特别是在水生物种中.
- 卡莱蒂库林在陆地哺乳动物中的抗菌作用在很大程度上仍未被探索.
研究的目的:
- 调查卡莱蒂库林在陆地哺乳动物中的潜在抗菌活性.
- 在山羊模型中评估calreticulin对细菌呼吸道感染的疗效.
主要方法:
- 用Pichia pastoris系统来表达和净化复合山羊卡莱蒂库林.
- 在体外测试中评估了calreticulin对大肠杆菌,沙门氏菌 typhimurium和帕斯泰雷拉多种菌的抑制活性.
- 在新生山羊中使用一种体内鼻腔感染模型,这些新生山羊受到Pasteurella multocida的挑战,以评估calreticulin的保护作用.
主要成果:
- 再组合的山羊和人类卡莱蒂库林在测试的细菌物种上表现出抑制作用.
- 发现calreticulin与细菌和细菌脂聚糖 (LPS) 结合,在Ca2+的存在下诱导聚合.
- 内注射卡莱蒂库林在感染帕斯图雷拉多种菌的山羊中降低了病理损伤和细菌负载,在呼吸道组织中观察到高调的内源卡莱蒂库林.
结论:
- 在哺乳动物模型中,calreticulin对关键细菌病原体表现出显著的抗菌活性.
- 卡尔雷蒂库林作为一种天然的免疫效应剂,可能通过抑制细菌的生长和传播而起作用.
- 这些发现支持开发基于calreticulin的,针对呼吸道感染的宿主向抗微生物策略.
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