从有机番茄中分离出来的一种新型GABA产生Levilactobacillus brevis菌株作为有希望的益生菌

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Asia Pizzi¹, Carola Parolin¹, Davide Gottardi²

¹Department of Pharmacy and Biotechnology, University of Bologna, 40127 Bologna, Italy.

Biomolecules

|July 29, 2025

概括

这项研究发现,从有机番茄中分离出来的Levilactobacillus brevis CRAI有效地产生胺黄油酸 (GABA). 这种乳酸细菌还表现出益生菌的潜力,使其适合功能性食品.

科学领域:

微生物学微生物学
食品科学食品科学食品科学
神经科学是一个神经科学.

背景情况:

胺黄油酸 (GABA) 是一种关键的神经递质,影响中枢神经系统和肠-大脑轴.
乳酸细菌 (LAB) 越来越多地被认为具有产生GABA等生物活性化合物的潜力.
有机番茄作为一个潜在的来源,以隔离具有GABA生产能力的新型LAB菌株.

研究的目的:

研究来自有机番茄的LAB产生GABA的能力.
为了识别和描述具有高GABA生产潜力的LAB菌株.
评估有前途的GABA生产菌株的益生菌性质和安全性.

主要方法:

使用16S rRNA基因测序从有机番茄中分离和分类学识别LAB.
通过HPLC检测谷氨酸脱碳酶基因 (gadB) 和量化GABA生产.
基因组测序,基因表达分析 (gadA,gadB) 和功能益生菌检测 (抗菌,抗炎,粘附).

主要成果:

在优化条件下,Levilactobacillus brevis CRAI在4%的单谷氨酸 (MSG) 中表现出最高的GABA产量.
基因组测序证实了L. brevis CRAI中gadA和gadB基因的存在,在MSG的存在下观察到上调.
L. brevis CRAI对肠道病原体表现出显著的抗微生物活性,减少了巨细胞中氧化的产生,并附着在肠道细胞中.

结论:

L. brevis CRAI 是生产GABA,一个关键的神经递质的有希望的候选者.
该菌株具有显著的益生菌特性,包括抗微生物和抗炎作用.
L. brevis CRAI有可能开发GABA丰富的功能性食品和益生菌补充剂来调节肠-大脑轴.

关键词:

这种细菌是Levilactobacillus brevis.它具有抗炎活性,具有抗炎活性.抗微生物活性的抗微生物活性.没有细胞的超生体.胺黄油酸 (GABA) 是一种胺黄油酸.益生菌是一种益生菌.

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