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Khald Blau^1,2, Claudia Gallert¹

¹Department of Microbiology-Biotechnology, Faculty of Technology, University of Applied Sciences Emden/Leer, Constantiaplatz 4, 26723 Emden, Germany.

Antibiotics (Basel, Switzerland)

|July 29, 2025

概括

移动遗传元素如转子体和质粒在环境Clostridioides difficile菌株中很普遍. 这些元素携带抗微生物耐药性基因,突出显示了这一One Health病原体潜在的水平基因转移.

科学领域:

微生物学微生物学
基因组学就是基因组学.
一个健康,一个健康.

背景情况:

困难菌是一种重要的病原体,对人类和动物健康都有影响.
已知移动遗传元素 (MGE) 在临床C. difficile菌株中存在.
在环境中C. difficile菌株中MGE的表征是有限的.

研究的目的:

确定MGEs的流行,包括可调动的转位子 (MTns),结合性转位子 (CTns),等离子体和插入序列.
分析环境中C. difficile分离物的全基因组序列 (WGS) 中的MGE.

主要方法:

对来自环境C. difficile菌株的166个WGS进行分析.
使用生物信息工具识别MGE.

主要成果:

48.2%的基因组含有九种类型的转子子.
在特定的C. difficile序列类型 (ST11,ST3,ST35) 中经常发现MTns和CTns.
抗微生物耐药性基因 (例如,tetM, ermB) 与CTns相关.
24.7%的基因组与等离子体组联系在一起,其中一些含有prophages.

结论:

环境C. difficile菌株含有丰富的等离子体,可能影响细菌生理学.
密切相关的CTns表明,C. difficile的抗微生物耐药性具有显著的水平转移.
需要进一步的研究来了解MGEs及其对C. difficile生物学的可转移性的影响.

关键词:

克洛斯特里迪奥伊德斯困难菌抗微生物耐药性抗微生物耐药性移动的遗传元素是移动的.塑料体中的塑料.转位子转位子

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