一个协同使用的MS平台,用于同时测定尿液中马隆迪化物和二酸
Gabriela Chango1, Diego García-Gómez1, Carmelo García Pinto1
1Universidad de Salamanca, Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemical Sciences, 37008 Salamanca, Spain.
概括
这项研究开发了一种新的方法来测量氧化应激 (甲) 和尿液中的有机酸盐 (二酸) 暴露. 研究结果表明,这两者之间存在联系,突出了阻燃剂暴露的潜在健康风险.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 生物标志物 生物标志物
背景情况:
- 氧化应激与各种健康问题有关.
- 有机酸盐阻燃剂,如三酸 (TPhP),是环境污染物.
- 评估暴露和生物影响需要精确的分析方法.
研究的目的:
- 开发和验证一种先进的分析方法,用于同时量化人类尿液中的甲 (MDA) 和二酸 (DPhP).
- 调查氧化应激生物标志物和TPhP暴露代谢物之间的相关性.
- 提供一种生物监测环境污染物和相关健康风险的工具.
主要方法:
- 同时量化使用水友互动液态色谱 (HILIC) 进行MDA和在线限制访问材料 (RAM) 进行DPhP.
- 使用经过认证的尿样进行方法验证,以确保稳定性和准确性.
- 72个人类尿样的高通量分析.
主要成果:
- 在人类尿液中MDA和DPHP水平之间发现了显著的正相关性 (ρ = 0.702,p < 0.001).
- 在女性中观察到适度的正相关性 (ρ = 0.622,p = 0.020),尽管样本规模小.
- 该方法在复杂的生物矩阵中表现出高的特异性,灵敏性和稳定性.
结论:
- 开发的分析方法对氧化应激和TPhP暴露的同时生物监测是有效的.
- 研究结果表明,氧化应激和暴露于TPhP之间存在潜在的联系.
- 这项研究提供了有关暴露的健康风险和环境污染物影响的宝贵见解.
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