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在噪音引起的听力损失患者中确定差异化miRNA表达特征

Gözde Öztan¹, Halim İşsever², Özlem Kar Kurt³

¹Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Topkapı, 34093 Istanbul, Turkey.

International journal of molecular sciences

|July 29, 2025

概括

循环中的microRNAs (c-miRNAs) 在噪音引起的听力损失 (NIHL) 患者中显示出明显的表达模式. 这些分子变化可以作为诊断和预测NIHL进展的生物标志物.

科学领域:

生物化学生物化学
遗传学是一个遗传学.
耳鼻喉科耳鼻喉科耳鼻喉科

背景情况:

噪音引起的听力损失 (NIHL) 是一种普遍的职业危险,导致耳损伤.
循环的microRNAs (c-miRNAs) 与细胞压力和内耳条件有关.
在NIHL病原体中c-miRNAs的特定作用尚不清楚.

研究的目的:

在NIHL患者中研究c-miRNAs的差异表达.
为了确定NIHL诊断和预后的潜在c-miRNA生物标志物.
探索c-miRNAs在NIHL相关的细胞机制中的参与.

主要方法:

来自NIHL患者 (n=12) 和对照组 (n=6) 的外周血液样本使用GeneChip® miRNA 4.0阵列进行了分析.
使用转录组分析控制台软件进行了差异表达式分析.
生物信息工具 (TargetScan,Enrichr) 预测了基因标和丰富的途径.

主要成果:

鉴定了72种差异表达的miRNA (FDR<0.05).
特定的miRNAs (例如,hsa-miR-486-2,hsa-miR-664b-3p) 被上调,而其他 (例如,hsa-miR-6723,hsa-miR-194-2) 被下调.
丰富分析表明它们在细胞亡,线粒体稳定性和细胞周期控制中的作用.

结论:

改变的c-miRNA配置文件与NIHL中的细胞应激和退行相关.
这些c-miRNAs代表了NIHL诊断和预后的潜在生物标志物.
需要进一步的功能和纵向研究来验证这些发现.

关键词:

这是一个miRNA数组阵列.这就是miRNAs.噪音引起的听力损失病变的发生和发病.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...