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饮食发酵与乳杆菌sp. 和Bacillus sp. 这两种类型. 在Bos taurus中调节体转录和微生物群概况

Jeong Sung Jung¹, Dahye Kim², Anand Singaravelu³

¹Forage Production System Division, National Institute of Animal Science, RDA, Cheonan 31000, Republic of Korea.

International journal of molecular sciences

|July 29, 2025

概括

发酵动物料使用乳酸菌和菌改变了牛的基因表达.

科学领域:

动物营养和新陈代谢
流体微生物学流体微生物学
基因表达分析基因表达分析

背景情况:

使用Lactobacillus和Bacillus发酵动物料正在引起人们的注意.
关于代谢组织和肠微生物中差异表达基因 (DEGs) 之间的相互作用的知识有限.
了解这些相互作用对于优化动物健康和表现至关重要.

研究的目的:

研究正常料 (NF) 与替代发酵料 (AF) 对Bos Taurus脂肪,肝脏和肌肉组织基因表达的影响.
分析NF和AF饮食对体微生物种群 (细菌和真菌) 的影响.
探索在关键代谢器官中肠微生物和DEG之间的关系.

主要方法:

使用高通量测序来分析基因表达和微生物种群.
在牛的NF和AF饮食之间进行了比较分析.
鉴定和量化了差异表达基因 (DEG) 和微生物丰度.

主要成果:

在脂肪 (34个基因),肝脏 (36个基因) 和肌肉 (28个基因) 组织中观察到显著的基因表达差异,主要与骨质细胞分化和免疫功能有关.
AF饮食显著改变了小的微生物组成,增加了Firmicutes,减少了Bacteroidetes的丰度.
特定的细菌系 (Faecalicatena,Intestinimonas,Lachnoclostridium) 和真菌系 (Orpinomyces,Piromyces) 受到AF饮食的显著影响,而Neocallimastix则下降.

结论:

替代发酵料可以调节牛的关键代谢组织中的基因表达.
AF饮食促进了更有利的小肠微生物平衡,可能提高动物的表现.
这项研究提供了关于饮食,宿主基因表达和肠微生物组之间的复杂相互作用的见解.

关键词:

巴西勒斯种类 (Bacillus sp.) 的存在博斯-金牛座 (Bos-taurus) 是一个巨大的象.乳酸菌种类 (Lactobacillus sp.) 的一个.发酵料发酵料是一种发酵料.微生物的动力学转录组 (transcriptome) 是一个转录组.

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