在DITRA中解码非编码RNA调节器:从基因组洞察到潜在的生物标记物和治疗点
Sofia Spanou1, Athena Andreou1,2, Katerina Gioti2
1Laboratory of Genetics, Department of Biotechnology, Agricultural University of Athens, 11855 Athens, Greece.
Genes
|July 29, 2025
概括
这项研究探讨了IL-36受体对手缺乏症 (DITRA) 中的非编码RNA,确定了这种罕见的自身炎症性疾病的TINCR和HNF4A等潜在生物标志物和治疗点.
科学领域:
- 遗传学和分子生物学
- 免疫学 免疫学 免疫学
- 系统生物学 系统生物学
背景情况:
- 缺少IL-36受体对手 (DITRA) 是一种罕见的单源性自身炎症性疾病.
- DITRA的特点是IL-36信号失调,是一般性性牛皮的亚型.
研究的目的:
- 调查IL36RN相互作用体内编码和非编码RNA (ncRNA) 的作用.
- 为了确定潜在的致病机制,生物标志物和治疗点DITRA.
主要方法:
- 一种系统生物学方法,利用STRING数据库来构建蛋白质-蛋白质相互作用网络.
- 通过RNAInter识别ncRNA相互作用,并使用Cytoscape进行网络分析.
- 路径丰富分析以确定候选ncRNA和基因的生物相关性.
主要成果:
- 鉴定出38种ncRNA,包括6种lncRNA和32种miRNA,与IL36RN网络相互作用.
- 33个ncRNA与DITRA相关的信号通路相关;七个蛋白质编码基因被突出显示,其中TINCR,PLEKHA1和HNF4A直接参与.
- 许多已识别的ncRNA与像牛皮这样的免疫媒介疾病有先前的关联,这表明它与DITRA相关.
结论:
- 提供了对DITRA中IL36RN的ncRNA介导调节的新见解.
- 特定的ncRNA和基因 (TINCR,PLEKHA1,HNF4A) 显示出作为DITRA的关键基因组元素的潜力.
- 结果可能会为生物标志物发现和针对DITRA的向治疗开发提供信息.
更多相关视频
06:43A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
Published on: August 31, 2013
18.8K
12:24DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
16.9K
相关概念视频
02:15Experimental RNAi
6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
01:29Translational Regulation
98
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
98
01:23Types of RNA
64.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
64.9K
02:39lncRNA - Long Non-coding RNAs
8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K
02:13Cooperative Binding of Transcription Regulators
6.6K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.6K
02:02Cis-regulatory Sequences
10.1K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
10.1K