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转录基因分析揭示了益生菌在调节猪毛囊发育中的分子机制

Yubin You¹, Beibei Han¹, Qiang He¹

¹State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

|July 29, 2025

概括

益生菌 (PRL) 通过增强颗粒细胞的增殖和血管生成,影响猪毛囊的生长. 这种激素影响像Hippo,JAK/STAT和Rap1这样的关键通路,对生殖健康至关重要.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
分子生物学分子生物学

背景情况:

益生菌 (PRL) 是一种重要的生殖激素.
它在猪毛囊发育中的确切作用尚未完全理解.

研究的目的:

研究PRL在猪毛囊发育中的作用.
探索PRL对粒粉细胞 (GC) 增殖,类固醇生成和血管生成的影响.

主要方法:

在体内分析PRL水平和不同大小的猪毛囊中的基因/蛋白质表达.
使用重组猪PRL (prPRL) 进行体外实验,以评估对GC增殖,类固醇生成和血管生成的影响.
使用定量实时PCR (RT-qPCR),西部抹杀 (WB),CCK-8测定和转录组测序.

主要成果:

在较大的猪毛囊中,PRL度更高.
在毛囊发育过程中,PRL及其受体 (PRLR) 表达,以及蛋白质分解酶和血管生成因子显示出动态变化.
重组prPRL促进了GC增殖,类固醇生成 (上调CYP11A1和3β-HSD) 和血管生成 (增强VEGFA和FGF-2).
转录组分析显示,在Hippo,JAK/STAT和Rap1信号通路中丰富的差异性基因表达.

结论:

在调节猪毛囊发育方面,PRL发挥着重要作用.
PRL影响GC增殖和血管生成.
PRL的影响通过Hippo,JAK/STAT和Rap1信号通路进行介导.

关键词:

血管新生的产生.毛囊的发展毛囊的发展细胞颗粒状颗粒素的细胞.益生菌蛋白是一种益生菌素.它们的扩散和扩散.

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