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通过下一代测序分析对周围病变的分类学多样性和临床相关性
Juliana D Bronzato1, Brenda P F A Gomes1, Tsute Chen2
1Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba 13083-970, SP, Brazil.
Genes
|July 29, 2025
概括
周周病变中的微生物多样性因地理和临床因素而异. 这项研究分析了下一代测序数据,以揭示与不同种群和症状相关的不同细菌概况.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 牙周内科医院 牙周内科医院 牙周内科医院
背景情况:
- 牙周周周病变的内牙起源的港湾复杂的微生物群落.
- 了解这些微生物群的分类学多样性和变异性对于有效的治疗策略至关重要.
研究的目的:
- 评估与周周病变相关的微生物群的分类学多样性.
- 为了确定微生物特征是否在不同种群和临床特征之间有所不同.
- 执行下一代测序 (NGS) 数据的统一的分析.
主要方法:
- 从三项已发表的研究中获取并重新处理原始的16S rRNA测序数据.
- 使用DADA2进行倍数序列变异推断和BLASTN进行分类学赋值.
- 使用QIIME 2和R进行多样性分析 (alpha和beta),通过ANCOM-BC2.2评估差异丰度.
主要成果:
- 38个周周病变样本产生了566,223个读数,分配给347种细菌物种.
- 根据地理区域,性别和症状观察到微生物组成的显著差异.
- 核心物种如 *Fretibacterium* sp. 这样的. 在研究中,HMT 360和*Porphyromonas endodontalis*是普遍存在的;*Porphyromonas gingivalis*和*Fusobacterium nucleatum*是大量存在的.
结论:
- 周周病变的微生物群在分类学上是多样化的.
- 微生物概况根据地理来源和临床特征,包括症状学,有很大差异.
- 症状性病变显示出 *Alloprevotella tannerae* 和 *Prevotella oris* 的丰度较高.
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