转录基因组剖析揭示了彩虹鱼蛋之间的差异,这些蛋具有高和低的生育潜力
Konrad Ocalewicz1, Artur Gurgul2, Stefan Dobosz3
1Department of Marine Biology and Biotechnology, Faculty of Oceanography and Geography, University of Gdańsk, M. Piłsudskiego 46 Av, 81-378 Gdynia, Poland.
Genes
|July 29, 2025
概括
彩虹鱼蛋中的母性基因表达影响了妇产遗传发育的成功. 转录的差异与双倍的单 haploid 存活率相关,这表明早期死亡的原因超出了胚胎基因表达.
科学领域:
- 水产养殖和生殖生物学
- 基因组学和分子生物学
- 发展生物学 发展生物学
背景情况:
- 妇产遗传双倍单体 (DHs) 是完全同卵性个体,对遗传研究至关重要.
- 鱼类DH的低生存率往往归因于衰退基因表达和子细胞治疗的副作用.
- 母亲基因产物显著影响早期胚胎发育,影响妇产生成效率.
研究的目的:
- 为了研究彩虹鱼卵的转录差异,具有不同的妇产遗传发育潜力.
- 为了确定影响妇产遗传双倍单 haploid (DHs) 的生存的母性因素.
- 利用RNA测序来理解与卵子对妇产生成能力相关的基因表达.
主要方法:
- 从八个不同的雌性中诱导彩虹鱼蛋中的妇产遗传发育.
- 从卵子中分离并对母体RNA进行RNA测序 (RNA-seq).
- 对妇产胚胎存活率和卵子转录组概况的比较分析.
主要成果:
- 在不同雌性卵子之间观察到显著的转录变异.
- 卵子的转录形状与它们成功发生妇产的能力有很强的相关性.
- 高存活度的DH卵中的上调基因与发育,细胞分化,迁移和蛋白质运输有关,包括卵细胞成熟和早期胚胎发生的基因.
结论:
- 母性基因转录的个体间变异与卵子对生殖的能力有关.
- 彩虹鱼DH死亡的一部分发生在胚胎基因组激活之前.
- 孕产妇的影响在妇产遗传鱼的早期生存中起着至关重要的作用.
相关概念视频
02:27Background and Environment Affect Phenotype
6.7K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.7K
02:24Ribosome Profiling
3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K