使用CFD建模,描述植物细胞悬浮物体体积对水力动力学的影响
Vidya Muthulakshmi Manickavasagam1, Kameswararao Anupindi2, Nirav Bhatt1,3
1Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, Tamil Nadu, India.
Biotechnology journal
|July 29, 2025
概括
植物细胞培养中的生物质生产率在扩展过程中经常丢失. 这项研究表明,维持恒定的剪切环境,而不是氧气转移,是成功培养生物反应器的关键.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 植物细胞培养植物细胞培养
背景情况:
- 由于水力动力学变化,植物细胞培养中的生物质生产率很难在生物反应器中复制.
- 摇瓶培养物经常被用作生物质生产率的基准.
研究的目的:
- 了解震动瓶体形状中的水力动力学变化与体积.
- 确定适合植物细胞培养的扩大标准.
- 为了最大限度地降低植物细胞培养规模的成本和时间.
主要方法:
- 利用计算流体动力学 (CFD) 和实验栽培.
- 研究了Viola odorata细胞培养在不断增加的瓶体积 (100-3000毫升) 中.
- 分析了体积氧质量转移系数 (kLa) 和能量消耗率.
主要成果:
- 生物质生产率在不同的瓶装量中保持一致.
- 体积氧质量转移系数 (kLa) 最初增加,然后随体积而下降.
- 恒定剪切环境,以每单位功率体积和表示,作为一个潜在的扩展参数而出现.
结论:
- 体积氧质量转移系数 (kLa) 可能不是Viola odorata的关键升级参数.
- 作为一个合适的扩展标准,建议保持恒定的剪切环境.
- 在生物反应器中最大限度地降低速度梯度,可以在扩展过程中提高生物质生产率.
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