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相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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单样,多原子质谱法用于研究药物效应和机制.

Iqbal Mahmud1, Wai-Kin Chan1, Karen Yannell2

  • 1Metabolomics Core Facility, Department of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, United States.

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概括

这项研究开发了一种多原子方法来分析药物毒性,揭示了l-asparaginase (ASNase) 影响抗氧化和炎症途径. 这种方法可以产生新的假设来减少药物的副作用.

关键词:
生物LC-MS 生物LC-MS 生物作为一种药物,它具有性酶.自动分成部分的分成部分.脂质组的类型是什么代谢生物组的代谢生物组多种多种多种多种多种多种多种多种多种多种.蛋白质组学 蛋白质组学毒性的毒性 毒性的毒性

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科学领域:

  • 生物化学 生物化学
  • 药理学 药理学是指药理学的学科.
  • 毒理学 毒理学 毒理学

背景情况:

  • 开发过程中的药物消耗通常是由于治疗指数差而引起的.
  • 鉴定药物毒性的机制对于药物开发至关重要.
  • 用于儿科急性淋巴细胞白血病的l-asparaginase (ASNase) 在成年人中具有毒性限制.

研究的目的:

  • 开发和验证一种用于阐明药物毒性机制的多原子方法.
  • 在免疫功能受损的小鼠中对l-asparaginase (ASNase) 的反应进行分析.
  • 为了产生减少ASNase毒性的假设,同时保持有效性.

主要方法:

  • 在小鼠中进行亚毒和有毒剂量的ASNase的纵向分析.
  • 采集了20μL的全血样本,加工成血,并提取了代谢物,脂质和蛋白质.
  • 利用三重四极体LC-MS/MS平台对500多种代谢物,750多种脂质和375种进行向的代谢学,脂质学和蛋白质学分析.

主要成果:

  • 确定了代谢产物,脂质和的剂量依赖调节.
  • 在抗氧化,炎症,自和细胞死亡途径上观察到调制分子的融合.
  • 证明了多原子工作流程产生关于ASNase毒性的新假设的能力.

结论:

  • 开发的三合一LC-MS/MS工作流对于有针对性的多原子分析是有效的.
  • ASNase毒性似乎与抗氧化,炎症,自和细胞死亡途径有关.
  • 抑制这些途径可能提供一种降低ASNase毒性和改善其治疗指数的策略.