相关实验视频

Updated: Aug 4, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

使用FPBG-PITC进行氨基酸含有代谢物的查,用于乳腺疾病的高吞吐量平台

Hongtao Tian¹, Wenjia Zhang², Yunhua Chen³

¹Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing, 100005, China.

|July 29, 2025

概括

一个新的磁探增强了血清氨基代谢物分析,用于早期发现乳腺疾病. 这种高通量方法可以识别潜在的生物标志物,改进诊断策略.

科学领域:

生物化学生物化学
分析化学分析化学
生物标志物发现发现

背景情况:

含氨基酸的代谢物对生物功能至关重要;它们的失调与疾病有关.
液体活检提供了非侵入性癌症检测,但在灵敏度和吞吐量方面面临挑战.
目前的方法需要大量的样本处理,限制了效率.

研究的目的:

开发一种新的,高吞吐量平台,用于分析含有血清氨基酸的代谢物.
使用专用磁探测器提高代谢物检测的灵敏度和效率.
为了确定早期乳腺疾病查的潜在生物标志物.

主要方法:

合成了一种具有疏水标签的新型磁性化学选择探头 (Fe3O4-聚合物-胺-博克利斯--NH2-PITC,FPBG-PITC).
建立一个高通量代谢分析平台,用于代谢物缩,净化和量化.
来自健康个体和患有乳腺病变的患者的血清样本的比较分析.

主要成果:

FPBG-PITC探针促进了血清氨基代谢产物的有效丰富和定量.
建立了一个高吞吐量平台,降低了成本并提高了分析性能.
四种代谢物 (阿斯巴拉金,氨酸,氨酸/异氨酸,homocitrulline) 被确定为潜在的早期乳腺疾病生物标志物.

结论:

开发的平台为血清氨基代谢物分析提供了一种新的,具有成本效益的策略.
已识别的代谢物显示出作为乳腺疾病早期检测的生物标志物具有前途.
这种方法推进了癌症诊断中的液体活检应用.

关键词:

含有氨基酸的代谢物.乳腺疾病乳腺疾病化学选择探针的探针.具有高通量功率的高通量功率.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量

更多相关视频

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases

Published on: May 17, 2019

相关概念视频

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...

Serum Laboratory Studies, Stool Test, Breath Test

Serum Laboratory Studies, Stool Test, Breath Test

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...

Endoscopic Procedures V: ERCP

Endoscopic Procedures V: ERCP

Endoscopic Retrograde Cholangiopancreatography (ERCP) is a diagnostic procedure that combines endoscopy and fluoroscopy to diagnose and treat conditions related to the bile ducts, pancreatic ducts, and gallbladder. This procedure is beneficial for identifying and addressing blockages, gallstones, strictures, and tumors within the biliary or pancreatic systems. ERCP is both diagnostic and therapeutic, offering the ability to visualize and treat identified problems in one session.
Patient...