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Updated: Sep 13, 2025

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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探索宏分子拥挤对4.1G-CTD的形状和NuMA结合的影响

Shi Yu1, Ming Yang2,3, Sarah Perrett2,4

  • 1Department of Chemistry, Institute of Biomedical Sciences and Multiscale Research Institute of Complex Systems, Fudan University, Shanghai 200438, China. wnwang@fudan.edu.cn.

Physical chemistry chemical physics : PCCP
|July 29, 2025
PubMed
概括

大分子拥挤压缩蛋白质 4.1G

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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
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