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单细胞5微米分辨率双极性MALDI-MS成像,没有矩阵重新应用

Yanyan Chen^1,2, Rui Shi¹, Jianing Wang^1,3

¹State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong SAR 315200, China.

Analytical chemistry

|July 29, 2025

概括

这项研究引入了一种新的双极性矩阵辅助激光吸附和电离质谱成像 (MALDI-MSI) 方法,用于单细胞脂质分析. 该技术实现了5μm分辨率,增强了脂管学研究和疾病机制研究.

科学领域:

分析化学分析化学
生物化学生物化学
分子成像学分子成像学

背景情况:

高分辨率质谱成像 (MSI) 对脂管学至关重要,但由于空间分辨率和脂质覆盖范围的限制,在单细胞分析中面临挑战.
现有的双极性MSI方法提供良好的脂质覆盖,但仅限于10微米分辨率,阻碍单细胞应用.

研究的目的:

为双极性MALDI-MSI开发一个单细胞/亚细胞分辨率战略.
为了克服当前MSI技术对细胞脂管学的空间分辨率限制.

主要方法:

一种新的单沉积矩阵应用技术,使用N-(1-naphthyl) -乙烯基二胺二化物 (NEDC) 进行双极性MALDI-MSI.
优化了采集参数和微调的矩阵沉积,用于5μm的空间分辨率,无需重新应用矩阵.
对小鼠脏组织和A549肺癌细胞的分析.

主要成果:

实现了5μm的空间分辨率,使单细胞和亚细胞脂质成像成为可能.
保持了与多步骤方法相比较的广泛脂质覆盖.
通过空间PLSA和组图分析,在单细胞种群中确定了明显的脂质分布模式和显著的细胞对细胞脂质异质性.

结论:

开发的单细胞分辨率MALDI-MSI战略克服了传统的双极性MSI的局限性.
为推进细胞脂管学,了解疾病机制和调查环境毒理学提供了强大的工具.

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