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在卵巢癌患者的肝瘤衍生生长因子和非编码RNA网络

Reza Ganjali¹, Setare Nassiri², Narges Zamani³

¹Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Biochemistry and biophysics reports

|July 29, 2025

概括

卵巢癌 (OC) 的进展与LINC00839-miR-345-5p-HDGF轴有关. 这项研究确定了肝瘤衍生生长因子 (HDGF) 作为OC的潜在生物标志物,并建议针对新疗法的lncRNAs和miRNAs.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

卵巢癌 (OC) 是一种致命的妇科恶性瘤,诊断生物标志物有限.
肝瘤衍生生长因子 (HDGF) 是一种涉及瘤进展的瘤基因,但其在OC中的作用尚未得到充分研究.
长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 可能通过竞争的内源RNA (ceRNA) 网络调节致癌途径.

研究的目的:

调查lncRNA LINC00839,miR-345-5p和HDGF在卵巢癌中的调节作用.
探索LINC00839-miR-345-5p-HDGF轴在OC病变发生中的潜力.
评估HDGF作为潜在的卵巢癌诊断生物标志物.

主要方法:

定量实时PCR (qRT-PCR) 用于分析50个OC和50个正常卵巢组织中的HDGF,LINC00839和miR-345-5p表达.
生物信息工具被用来预测RNA-RNA相互作用,并构建一个ceRNA网络.
进行了统计分析,包括相关性分析和接收器运行特征 (ROC) 曲线分析.

主要成果:

在OC组织中,HDGF和LINC00839被显著上调,而miR-345-5p被降调 (p<0.001).
HDGF表达与LINC00839正相关,与miR-345-5p相反,表明一个ceRNA调节轴.
升高的HDGF水平与晚期瘤阶段,较大的瘤大小和转移有关. HDGF 血清水平显示出有前途的诊断潜力 (AUC = 0.88).

结论:

LINC00839-miR-345-5p-HDGF轴是卵巢癌的一个关键调节网络.
HDGF可以作为OC进展的临床相关生物标志物.
在OC中,LINC00839和miR-345-5p代表了基于ceRNA的干预措施的潜在治疗点.

关键词:

肝瘤衍生生长因子的生长因子这里是 LINC0083939 的位置.卵巢癌是发生在卵巢中的癌症.在 miR-345-5p 中使用.在 miR-384 的位置上.

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