酸酸酶是通过在固体发酵中使用小米生产的
Jussara Maria Martins de Almeida Afonso1, Frederico Alves Lima1, Miriam Maria de Resende1
1Chemical Engineering Faculty, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Campus Santa Mônica, Bloco 1K, 38408-144 Uberlândia, MG, Brazil.
ACS omega
|July 29, 2025
概括
这项研究优化了使用小米的固态发酵来生产酸酸酶 (AcPases). 最好的条件产生了显著的酶活性和生物质,这对于有机利用至关重要.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 微生物生物技术 微生物生物技术
背景情况:
- 酸酶 (AcPases) 是有机吸收和利用的关键酶.
- 菌在通过酶性水解将不溶性转化为可溶性中发挥着关键作用.
- 在酸性pH值 (低于6.00) 的情况下,AcPases的功能最好.
研究的目的:
- 评估和优化酸酸酶的生产和提取.
- 为了研究使用小米作为基质的固态发酵 (SSF) 的潜力.
- 确定酶活性和生物质生产的最佳条件.
主要方法:
- 固态发酵 (SSF) 使用小米作为基质.
- 预先查以确定最佳基质和菌株潜力.
- 中央复合设计 (CCD) 优化变量:小米质量,注射剂度和湿度.
- 使用Tween 80进行提取.
主要成果:
- 小麦被确定为酶生产中最有效的基质.
- 使用CCD优化的SSF条件产生了显著的生物质 (9.27 ± 0.53 g/L) 和酸酸酶活性 (36.09 ± 0.61 U/mL).
- 在无菌蒸水中培养和提取Tween 80增强了结果.
结论:
- 用小米进行固态发酵是生产酸酸酶的有效方法.
- 优化条件显著增加了酶产量和生物质.
- 这一过程具有有效的有机管理的潜力.
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