Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Fermentation01:29

Fermentation

97.1K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
97.1K
Microbial Fermentation01:23

Microbial Fermentation

1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
Microbes in Food Production01:29

Microbes in Food Production

403
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
403
Microbes in Beverage Production01:25

Microbes in Beverage Production

298
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
298
Microbes in the Production of Fermented Foods01:27

Microbes in the Production of Fermented Foods

327
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
327
Production of Organic Acids01:25

Production of Organic Acids

105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spin Waves Excited by Hard X-Ray Transient Gratings.

Physical review letters·2026
Same author

Statistical optimization of acid phosphatase production by <i>Trichoderma</i> spp. in submerged fermentation.

3 Biotech·2026
Same author

Optimized GA<sub>3</sub> Production by <i>Aspergillus niger</i> and <i>Fusarium fujikuroi</i>: From Low-Cost Fermentation to Agronomic Application.

ACS omega·2026
Same author

Electronic Structure of Coenzyme B<sub>12</sub> Excited States as Probed by Time-Resolved X-ray Absorption and Emission Spectroscopies.

The journal of physical chemistry. B·2025
Same author

Insights into using <i>Kosakonia oryzae</i> AG15 in liquid formulations containing sodium alginate, glycerol, and gum arabic for the sustainable development of <i>Phaseolus vulgaris</i> L.

3 Biotech·2025
Same author

Application of Ion-Exchange Membranes With Graphene Oxide in the Removal of Metal Ions Using the Electrodialysis Process.

Water environment research : a research publication of the Water Environment Federation·2025

相关实验视频

Updated: Apr 30, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.1K

酸酸酶是通过在固体发酵中使用小米生产的.

Jussara Maria Martins de Almeida Afonso1, Frederico Alves Lima1, Miriam Maria de Resende1

  • 1Chemical Engineering Faculty, Federal University of Uberlândia, P.O. Box 593, Av. João Naves de Ávila 2121, Campus Santa Mônica, Bloco 1K, 38408-144 Uberlândia, MG, Brazil.

ACS omega
|July 29, 2025
PubMed
概括

这项研究优化了使用小米的固态发酵来生产酸酸酶 (AcPases). 最好的条件产生了显著的酶活性和生物质,这对于有机利用至关重要.

科学领域:

  • 生物化学 生物化学
  • 酶学 是一种酶学.
  • 微生物生物技术 微生物生物技术

背景情况:

  • 酸酶 (AcPases) 是有机吸收和利用的关键酶.
  • 菌在通过酶性水解将不溶性转化为可溶性中发挥着关键作用.
  • 在酸性pH值 (低于6.00) 的情况下,AcPases的功能最好.

研究的目的:

  • 评估和优化酸酸酶的生产和提取.
  • 为了研究使用小米作为基质的固态发酵 (SSF) 的潜力.
  • 确定酶活性和生物质生产的最佳条件.

主要方法:

  • 固态发酵 (SSF) 使用小米作为基质.
  • 预先查以确定最佳基质和菌株潜力.
  • 中央复合设计 (CCD) 优化变量:小米质量,注射剂度和湿度.
  • 使用Tween 80进行提取.

主要成果:

  • 小麦被确定为酶生产中最有效的基质.
  • 使用CCD优化的SSF条件产生了显著的生物质 (9.27 ± 0.53 g/L) 和酸酸酶活性 (36.09 ± 0.61 U/mL).
  • 在无菌蒸水中培养和提取Tween 80增强了结果.

更多相关视频

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

15.8K
Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

380

相关实验视频

Last Updated: Apr 30, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.1K
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
04:59

Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm

Published on: April 12, 2019

15.8K
Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
06:08

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

380

结论:

  • 用小米进行固态发酵是生产酸酸酶的有效方法.
  • 优化条件显著增加了酶产量和生物质.
  • 这一过程具有有效的有机管理的潜力.