微波辅助消化方法和分散磁性固相微提取,用于通过ICP-OES确定红细胞生物质中的主要元素和微量元素
Camilla M Belmiro1,2, Mikaelle de Carvalho Gomes1,2, Fernanda Nunes Ferreira1
1Departamento de Química Analítica, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, Rio de Janeiro, RJ 20550-013, Brazil.
ACS omega
|July 29, 2025
概括
这项研究提出了一种新方法,用于分析用微波辅助消化和磁性固相微提取用于基纤维素生物质的主要和微量元素. 该方法准确量化了生物质样本中的,铁,和等元素.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 精确的元素分析是关键的,以了解其组成和潜在的应用.
- 现有的方法可能需要复杂的样品制备或缺乏对微量元素的敏感性.
研究的目的:
- 开发和验证一种可靠的样本制备方法,用于确定基纤维素生物质中的主要元素和微量元素.
- 使用感应合等离子光学发射光谱 (ICP-OES) 进行敏感元素量化.
主要方法:
- 微波辅助消化 (MAD) 用酸作为主要元素.
- 分散磁性固体相微提取 (DMSPE) 使用合成的maghemite纳米颗粒用于微量元素 (As,Pb,Se).
- 使用中央复合设计优化MAD和DMSPE参数.
主要成果:
- 对于MAD (280毫克样品,2.5毫升HNO3,2.0毫升H2O2) 和DMSPE (10毫克吸附剂,100分钟,pH9.5) 实现了优化条件.
- 对于As (0.01μg g-1),Pb (0.03μg g-1),Se (0.01μg g-1) 的低检测极限 (LOQ) 进行检测.
- 微量元素的高丰富系数 (As:21,Pb:31,Se:42). 微量元素的高丰富系数 (As:21,Pb:31,Se:42). 微量元素的高丰富系数 (As:21,Pb:31,Se:42).
- 在五个不同的生物质样本中精确量化主要元素 (Ca,Cu,Fe,Mg,Mn,Na,Zn) 和微量元素.
结论:
- 拟议的方法提供了一种高效和准确的方法,用于在基纤维素生物质中进行元素分析.
- MAD和DMSPE的组合为各种各样的元素提供了一种多功能样本准备策略.
- 该方法适用于生物质样本的常规分析,有助于生物质价值化研究.
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