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长期单颗粒追踪膜蛋白由上转换性-地球纳米探测器实现

Huan Ling¹, Jinyang Liu¹, Fan Ding¹

¹Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China.

|July 29, 2025

概括

工程上转化纳米粒子使生物分子的单颗粒追踪能够扩展. 这种改进的方法揭示了明显的运动状态和细胞骨调节表皮生长因子受体动态.

科学领域:

生物物理学的生物物理.
纳米技术纳米技术
细胞生物学细胞生物学

背景情况:

单粒子追踪 (SPT) 提供了对生物分子机制的洞察力,但受到探头光稳定性的限制.
上转换SPT (uSPT) 使用化物合纳米粒子 (UCNPs) 进行更长的成像,但面临着尺寸亮度的权衡.

研究的目的:

开发更明亮,更小的UCNPs,以增强 uSPT.
通过 uSPT.研究表皮生长因子受体 (EGFR) 的动态和调节.

主要方法:

用基于的材料设计和合成了~10纳米核心--UCNP.
工程UCNPs与传统的基于的UCNPs的发光比较.
应用 uSPT 来跟踪细胞中的个体EGFR动态,并评估latrunculin A治疗的影响.

主要成果:

工程UCNPs显示亮度增加4.6倍,同时保持小尺寸.
确定了四种不同的EGFR运动状态:不动,受限,自由和定向.
在latrunculin A治疗后观察到EGFR运动状态的显著变化,涉及细胞骨.

结论:

新型的UCNP克服了SUPT的尺寸亮度限制.
uSPT成功地阐明了个别EGFR的异质动态和可逆转变.
这些发现强调了细胞骨在调节膜蛋白行为和细胞过程中的关键作用.

关键词:

这是性土壤.膜蛋白质是一种膜蛋白质.单颗粒追踪器是一个单颗粒追踪器.上升转换的发光效应

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