固体证据和液体黄金:处理固体,全流体或离心流体的交易,用于在废水中检测病毒,细菌和真核病原体
Sooyeol Kim1, Denise Garcia1, Caroline McCormack1
1Department of Civil and Environmental Engineering, University of California, Berkeley, Berkeley, California 94720, United States.
Environmental science & technology
|July 29, 2025
概括
统一的工作流可以同时检测到各种废水病原体 (病毒,细菌,真菌). 离心影响样本为广泛的病原体监测提供了固体的可行替代方案.
科学领域:
- 环境微生物学环境微生物学
- 分子诊断学 分子诊断学
- 公共卫生监督是对公共卫生的监督.
背景情况:
- 有效的废水病原体监测需要同时检测各种微生物病原体的方法.
- 目前的方法在样品类型和核酸提取方面面临挑战,影响广泛的病原体检测.
研究的目的:
- 调查样品类型和核酸提取对废水中病毒,细菌,原生动物和真菌病原体的同时检测的影响.
- 建立一个单一的工作流程,用于在废水中广泛检测病原体.
主要方法:
- 在废水固体和整个影响物中比较dPCR检测尖端和内源性标 (病毒,细菌,原生动物,真菌,ARG).
- 评估了三种样本类型:固体,离心流体和来自11个设施的整体流体.
- 通过使用PMMoV,carjivirus和16SrRNA评估正常化.
主要成果:
- 一个单一的工作流成功地同时检测出所有目标病原体.
- 检测率和核酸度在固体,离心影响和整个影响之间是相似的.
- 离心流入物显示的目标度略高,是可行的样本类型,绕过固体的停留时间限制.
结论:
- 一个统一的工作流允许同时检测各种废水病原体.
- 离心流入型是适合于废水中广泛的病原体监测的样品类型.
- 对于准确的度解释,需要仔细考虑规范化控制.
相关概念视频
Coagulation
373
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
373
Centrifugation
2.5K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
2.5K


