Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Physicians and artificial intelligence diverge in evaluating large language models on real clinical cases.

NPJ digital medicine·2026
Same author

Intracranial Pressure Reduction Is Associated with Mitochondrial OPA1 and Cytochrome c Release in the Retinas of AQP1-Null Mice.

Brain sciences·2026
Same author

Optic nerve intraneural pressure trend under intraocular-intracranial pressure effects.

The British journal of ophthalmology·2026
Same author

Bioengineered 3D Human Trabecular Meshwork Models for Outflow Physiology and Glaucoma Research.

Bioengineering (Basel, Switzerland)·2026
Same author

NRF2 Deficiency Disrupts Mitochondrial Homeostasis via NDUFS7 in Trabecular Meshwork.

Research (Washington, D.C.)·2026
Same author

Retinal vascular alteration following surgical intraocular pressure reduction in primary angle closure disease.

BMJ open ophthalmology·2026

相关实验视频

Updated: May 7, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
11:00

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Published on: January 12, 2015

11.7K

人类肌网干细胞和肌网细胞之间的转录基因差异揭示了特定的生物标志物配置文件.

Rong Du1,2, Ajay Kumar3, Enzhi Yang2

  • 1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing Key Laboratory of Intelligent Diagnosis Technology and Equipment for Optic Nerve-Related Eye Diseases, Capital Medical University, Beijing 100730, China.

Current issues in molecular biology
|July 29, 2025
PubMed
概括

脊椎网状干细胞 (TMSC) 和脊椎网状细胞 (TMC) 具有不同的分子配置. 识别这些差异为开发新型玻璃眼干细胞疗法提供了关键生物标志物.

关键词:
有关RNA测序的RNA测序玻璃眼 glaucoma 玻璃眼 玻璃眼 玻璃眼 玻璃眼核心基因 核心基因 核心基因微阵列的微阵列干细胞是干细胞的组成部分.状网状网状网状网状网状网状网.转录基因分析的概况

更多相关视频

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

11.0K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.4K

相关实验视频

Last Updated: May 7, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
11:00

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Published on: January 12, 2015

11.7K
In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

11.0K
Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.4K

科学领域:

  • 眼科医生 眼科 眼科
  • 再生医学是一种再生医学.
  • 基因组学就是基因组学.

背景情况:

  • 玻璃眼是导致不可逆转失明的主要原因.
  • 椎间板状网络 (TM) 功能障碍是青光眼的主要原因.
  • 脊柱状网状干细胞 (TMSCs) 显示出TM再生疗法的潜力,但它们的分子身份尚不清楚.

研究的目的:

  • 综合比较人类TMSCs和人类TM细胞 (TMCs) 的分子特性.
  • 识别TMSCs和TMCs的独特分子特征和潜在生物标志物.
  • 为未来基于干细胞的青光眼治疗奠定基础.

主要方法:

  • 用RNA测序和微阵列分析进行了转录基因比较.
  • 使用定量PCR (qPCR) 来验证基因表达.
  • 进行了功能丰富和网络拓分析,以解释基因表达数据.

主要成果:

  • 在TMSCs和TMCs之间,共有465个差异表达的基因被确定.
  • TMSCs在发育,免疫信号和细胞外矩阵重塑通路方面表现出丰富.
  • TMCs在结构,收缩和粘附相关的功能上得到了丰富,并确定了特定的枢纽基因.

结论:

  • 独特的分子特征区分TMSCs和TMCs.
  • 已识别的基因如CXCL3,CXCL6,BMP2,LMOD1和BGN作为细胞身份的可靠生物标志物.
  • 这些发现为开发针对性干细胞治疗青光眼的开发提供了基础.