临灭绝的药用植物Trichopus zeylanicus的完整线粒体基因组序列的组装和比较分析
Biju Vadakkemukadiyil Chellappan1, P R Shidhi2, Anu Sasi3
1Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi Arabia.
Current issues in molecular biology
|July 29, 2025
概括
我们对药用植物Trichopus zeylanicus的完整线粒体基因组进行了测序. 这项研究揭示了独特的基因安排和对单一细胞线粒体基因组的进化见解.
科学领域:
- 植物基因组学 植物基因组学
- 线粒体DNA的演化过程
- 药用植物的研究研究.
背景情况:
- 植物线粒体基因组显示出显著的尺寸和结构多样性.
- 特里科普斯 (Trichopus zeylanicus) 是一种来自西加特山脉的特有药用植物.
研究的目的:
- 报告Trichopus zeylanicus的完整的线粒体基因组序列.
- 分析其基因组结构,基因含量和单种动物中的进化关系.
主要方法:
- 使用Illumina短读和PacBio长读技术进行混合测序.
- 生物信息组装,注释和比较基因组分析.
- 使用线粒体基因进行的遗传学分析.
主要成果:
- 特里科普斯 (Trichopus zeylanicus) 的线粒基因组为709,127个基因组,其中GC含量为46%,编码32个蛋白质编码基因,17个tRNA和3个rRNA.
- 与其他单体植物相比,观察到显著的谱系特异性重新排列,将基因组大小与结构性保护脱.
- 遗传学分析将T. zeylanicus定位为Dioscoreales中的一个独特的血统.
结论:
- 这项研究为Dioscoreales序列提供了第一个线粒基因组资源.
- 它促进了对线粒体基因组多样性和单体动物进化的理解.
- 特里科普斯 (Trichopus zeylanicus) 呈现出独特的线粒体基因组特征,支持其进化特色.
相关概念视频
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
13.5K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
13.5K
02:54Evolutionary Relationships through Genome Comparisons
6.2K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.2K
02:19Export of Mitochondrial and Chloroplast Genes
3.8K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.8K
02:59Animal Mitochondrial Genetics
8.0K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
8.0K
01:29Modern Molecular Taxonomy
145
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
145