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孕产妇年龄对人类MII卵细胞转录组产生不一致的影响

Xiaorui Zhang¹, Jiao Yang¹, Wenting Yang¹

¹Department of Reproductive Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Molecular human reproduction

|July 29, 2025

概括

先进的母亲年龄会影响卵细胞基因表达,但签名是不一致的. 一项元分析确定了25个与年龄相关的基因,包括蛋白质体子单元,表明多因素卵细胞衰老具有有限的转录基因作用.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
衰老研究研究衰老研究

背景情况:

晚年母亲的年龄与卵细胞质量降低有关.
现有的研究表明,卵细胞基因表达的年龄相关变化,但对特定基因的共识有限.
了解这些转录组变化对于生殖健康至关重要.

研究的目的:

描述年龄对人类MII卵细胞转录组的影响.
为了识别可复制的与年龄相关的基因表达特征.
探索转录组变化在卵细胞衰老中的作用.

主要方法:

从年轻和年长的捐赠者生成单卵细胞Smart-seq2数据.
与12项先前的研究 (人类和小鼠) 进行了交叉引用.
实施了一种元分析方法,将新的和现有的卵细胞转录组数据 (共150个图书馆) 结合起来.

主要成果:

在数据集之间发现了相当大的不一致性,表明与年龄相关的基因表达特征的复制性不佳.
在多项研究中确定了40个具有年龄改变表达的基因,包括那些参与蛋白质稳定性的基因.
分析结果显示,25个基因与年龄有显著的相关性,其中14个基因通过RT-PCR验证,包括老卵细胞中下调的蛋白质体子单元 (PSMA1,PSMA2).

结论:

卵细胞衰老是一个多因素过程,其中转录基因变化起着有限的作用.
在转录中存在明显的卵细胞间异质性.
蛋白质组变化可能比以前认为的在卵细胞衰老中起到更重要的作用.

关键词:

不同的基因表达方式这是一个元分析.卵细胞衰老卵细胞衰老卵细胞质量卵细胞质量蛋白质酶体蛋白质组是什么

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